TY - JOUR
T1 - DAF-7/TGF-β expression required for the normal larval development in C. elegans is controlled by a presumed guanylyl cyclase DAF-11
AU - Murakami, Mayumi
AU - Koga, Makoto
AU - Ohshima, Yasumi
N1 - Funding Information:
We thank A. Fire for pPD95.77 vector and J.M. Kramer for pRF4. C. elegans strains were obtained from Caenorhabditis elegans Genetics Center, which is funded by the National Institute of Health Center for Research Resources. We also thank members of our laboratory for discussion and M. Ohara for language assistance. This research was supported by grants from the Ministry of Education, Science, Sports and Culture of Japan, by a grant from the Japan Science and Technology Corporation to M.K., and by Research for the Future 97L00401 from Japan Society for Promotion of Science.
PY - 2001
Y1 - 2001
N2 - In C. elegans development, unfavorable growth conditions lead a larva to an arrested and enduring form called a dauer. To elucidate components upstream of DAF-7/TGF-β in this control pathway, we isolated a mutant that was defective in daf-7 promoter::gfp reporter expression and showed an arrested (dauer-constitutive) phenotype. It has a new mutation in the daf-11 gene encoding a transmembrane guanylyl cyclase. We show that daf-11 gene and a related gene daf-21 act upstream of daf-7, and cilium-related genes che-2 and che-3 are placed between daf-11 and daf-7, in the genetic pathway controlling dauer formation. Expression of daf-11 cDNA by cell specific promoters suggests that daf-11 acts cell autonomously in ASI chemosensory neurons for daf-7 expression.
AB - In C. elegans development, unfavorable growth conditions lead a larva to an arrested and enduring form called a dauer. To elucidate components upstream of DAF-7/TGF-β in this control pathway, we isolated a mutant that was defective in daf-7 promoter::gfp reporter expression and showed an arrested (dauer-constitutive) phenotype. It has a new mutation in the daf-11 gene encoding a transmembrane guanylyl cyclase. We show that daf-11 gene and a related gene daf-21 act upstream of daf-7, and cilium-related genes che-2 and che-3 are placed between daf-11 and daf-7, in the genetic pathway controlling dauer formation. Expression of daf-11 cDNA by cell specific promoters suggests that daf-11 acts cell autonomously in ASI chemosensory neurons for daf-7 expression.
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U2 - 10.1016/S0925-4773(01)00507-X
DO - 10.1016/S0925-4773(01)00507-X
M3 - Article
C2 - 11677050
AN - SCOPUS:0034767550
SN - 0925-4773
VL - 109
SP - 27
EP - 35
JO - Mechanisms of Development
JF - Mechanisms of Development
IS - 1
ER -