D-RECS (3) development of functional polymers for inflammatory cells specific gene delivery system

Daisuke Asai, Yoko Shoji, Kenji Kawamura, Jun Oishi, Jeong Hun Kang, Yuko Sato, Takuro Niidome, Hideki Nakashima, Yoshiki Katayama

    Research output: Contribution to conferencePaperpeer-review

    Abstract

    Recently, we have developed and proposed a new system of the intracellular signal-responsive gene regulation as cell specific gene delivery, and termed the new concept D-RECS. In the present study, we focused IκB kinase that regulates NF-κB activation. According to D-RECS strategy, acrylamide polymers containing a substrate peptide for IκB kinase were designed and synthesized. The synthetic polymers were first examined in the gel retardation assay for IκB kinase β (IKKβ) using recombinant IKKβ. A polymer showed the efficient binding activity for plasmid DNA and significant responses to IKKβ. When the polymer-reporter plasmid DNA complex was transfected into living cells, and then the reporter activity was measured under the condition with or without TNFα stimulation, the gene expression with TNFα was stronger than that without TNFα stimulation. The differences in the reporter activity were found to be well-correlated to the TNFα stimulation.

    Original languageEnglish
    Pages1953
    Number of pages1
    Publication statusPublished - 2006
    Event55th SPSJ Annual Meeting - Nagoya, Japan
    Duration: May 24 2006May 26 2006

    Other

    Other55th SPSJ Annual Meeting
    Country/TerritoryJapan
    CityNagoya
    Period5/24/065/26/06

    All Science Journal Classification (ASJC) codes

    • General Engineering

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