Cytoplasmic ubiquitin ligase KPC regulates proteolysis of p27Kip1 at G1 phase

Takumi Kamura, Taichi Hara, Masaki Matsumoto, Noriko Ishida, Fumihiko Okumura, Shigetsugu Hatakeyama, Minoru Yoshida, Keiko Nakayama, Keiichi I. Nakayama

Research output: Contribution to journalArticlepeer-review

361 Citations (Scopus)


The cyclin-dependent kinase inhibitor p27Kip1 is degraded at the G0-G1 transition of the cell cycle by the ubiquitin-proteasome pathway. Although the nuclear ubiquitin ligase (E3) SCFSkp2 is implicated in p27Kip1 degradation, proteolysis of p27Kip1 at the G0-G1 transition proceeds normally in Skp2-/- cells. Moreover, p27Kip1 is exported from the nucleus to the cytoplasm at G0-G1 (refs 9-11). These data suggest the existence of a Skp2-independent pathway for the degradation of p27Kip1 at G1 phase. We now describe a previously unidentified E3 complex: KPC (Kip1 ubiquitination-promoting complex), consisting of KPC1 and KPC2. KPC1 contains a RING-finger domain, and KPC2 contains a ubiquitin-like domain and two ubiquitin-associated domains. KPC interacts with and ubiquitinates p27Kip1 and is localized to the cytoplasm. Overexpression of KPC promoted the degradation of p27Kip1, whereas a dominant-negative mutant of KPC1 delayed p27Kip1 degradation. The nuclear export of p27Kip1 by CRM1 seems to be necessary for KPC-mediated proteolysis. Depletion of KPC1 by RNA interference also inhibited p27Kip1 degradation. KPC thus probably controls degradation of p27Kip1 in G1 phase after export of the latter from the nucleus.

Original languageEnglish
Pages (from-to)1229-1235
Number of pages7
JournalNature Cell Biology
Issue number12
Publication statusPublished - Dec 2004

All Science Journal Classification (ASJC) codes

  • Cell Biology


Dive into the research topics of 'Cytoplasmic ubiquitin ligase KPC regulates proteolysis of p27Kip1 at G1 phase'. Together they form a unique fingerprint.

Cite this