TY - JOUR
T1 - Cytoplasmic fusion between an enlarged embryonic stem cell and a somatic cell using a microtunnel device
AU - Kim, Seong Min
AU - Wada, Ken Ichi
AU - Ueki, Masashi
AU - Hosokawa, Kazuo
AU - Maeda, Mizuo
AU - Sakai, Yasuyuki
AU - Ito, Yoshihiro
N1 - Funding Information:
We thank Mitchell Arico and Alison Sherwin, PhD, from Edanz Group ( www.edanzediting.com/ac ) for editing a draft of this manuscript. S.M.K. was supported by Junior Associate Program of RIKEN.
Publisher Copyright:
© 2019 Elsevier Inc.
PY - 2019/12/3
Y1 - 2019/12/3
N2 - Based on a previous finding that fusion of a somatic cell with an embryonic stem (ES) cell reprogrammed the somatic cell, genes for reprogramming transcription factors were selected and induced pluripotent stem (iPS) cell technology was developed. The cell fusion itself produced a tetraploid cell. To avoid nuclear fusion, a method for cytoplasmic fusion using a microtunnel device was developed. However, the ES cell was too small for cell pairing at the device. Therefore, in the present study, ES cell enlargement was carried out with the colchicine derivative demecolcine (DC). DC induced enlargement of ES cells without loss of their stemness. When an enlarged ES cell was paired with a somatic cell in the microtunnel device, cytoplasmic fusion was observed. The present method may be useful for further development of reprogramming techniques for iPS cell preparation without gene transfection.
AB - Based on a previous finding that fusion of a somatic cell with an embryonic stem (ES) cell reprogrammed the somatic cell, genes for reprogramming transcription factors were selected and induced pluripotent stem (iPS) cell technology was developed. The cell fusion itself produced a tetraploid cell. To avoid nuclear fusion, a method for cytoplasmic fusion using a microtunnel device was developed. However, the ES cell was too small for cell pairing at the device. Therefore, in the present study, ES cell enlargement was carried out with the colchicine derivative demecolcine (DC). DC induced enlargement of ES cells without loss of their stemness. When an enlarged ES cell was paired with a somatic cell in the microtunnel device, cytoplasmic fusion was observed. The present method may be useful for further development of reprogramming techniques for iPS cell preparation without gene transfection.
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U2 - 10.1016/j.bbrc.2019.09.131
DO - 10.1016/j.bbrc.2019.09.131
M3 - Article
C2 - 31594640
AN - SCOPUS:85073009263
SN - 0006-291X
VL - 520
SP - 257
EP - 262
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -