TY - JOUR
T1 - Cytokine-based high log-scale expansion of functional human dendritic cells from cord-blood CD34-positive cells
AU - Harada, Yui
AU - Okada-Nakanishi, Yae
AU - Ueda, Yasuji
AU - Tsujitani, Shunichi
AU - Saito, Satoru
AU - Fuji-Ogawa, Terumi
AU - Iida, Akihiro
AU - Hasegawa, Mamoru
AU - Ichikawa, Tomohiko
AU - Yonemitsu, Yoshikazu
N1 - Funding Information:
The authors would like to thank Drs. Mariko Yoshizaki, Akihiro Tagawa, Takumi Kanaya, Hiroshi Ban, and Takashi Hironaka for their excellent technical assistance with the construction and large-scale production of rSeV vectors. This work was supported in part by a Grant-in-Aid (to YY) from the Japanese Ministry of Education, Culture, Sports, Science, and Technology; and by Research Grants from the Sankyo Foundation of Life Science (to YY) and from the Uehara Memorial Foundation (to YY).
PY - 2011
Y1 - 2011
N2 - Dendritic cells (DCs) play a crucial role in maintaining the immune system. Though DC-based cancer immunotherapy has been suggested as a potential treatment for various kinds of malignancies, its clinical efficacies are still insufficient in many human trials. Issues that limit the clinical efficacy of DC-based immunotherapy, as well as the difficulty of the industrial production of DCs, are largely due to the limited number of autologous DCs available from each patient. We here established a possible breakthrough, a simple cytokine-based culture method to expand the log-scale order of functional human DCs. Floating cultivation of cord-blood CD34 + cells under an optimized cytokine cocktail led these progenitor cells to stable log-scale proliferation and to DC differentiation. The expanded DCs had typical features of conventional myeloid DCs in vitro. Therefore, the concept of DC expansion should contribute significantly to the progress of DC immunotherapy.
AB - Dendritic cells (DCs) play a crucial role in maintaining the immune system. Though DC-based cancer immunotherapy has been suggested as a potential treatment for various kinds of malignancies, its clinical efficacies are still insufficient in many human trials. Issues that limit the clinical efficacy of DC-based immunotherapy, as well as the difficulty of the industrial production of DCs, are largely due to the limited number of autologous DCs available from each patient. We here established a possible breakthrough, a simple cytokine-based culture method to expand the log-scale order of functional human DCs. Floating cultivation of cord-blood CD34 + cells under an optimized cytokine cocktail led these progenitor cells to stable log-scale proliferation and to DC differentiation. The expanded DCs had typical features of conventional myeloid DCs in vitro. Therefore, the concept of DC expansion should contribute significantly to the progress of DC immunotherapy.
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U2 - 10.1038/srep00174
DO - 10.1038/srep00174
M3 - Article
C2 - 22355689
AN - SCOPUS:84859746200
SN - 2045-2322
VL - 1
JO - Scientific reports
JF - Scientific reports
M1 - 174
ER -
