TY - JOUR
T1 - Cytochrome b558, a component of the phagocyte NADPH oxidase, is a flavoprotein
AU - Sumimoto, Hideki
AU - Sakamoto, Norihiro
AU - Nozaki, Masahiko
AU - Sakaki, Yoshiyuki
AU - Takeshige, Koichiro
AU - Minakami, Shigeki
N1 - Funding Information:
Acknowledgment--This work was supported in part by grants from the Ministry of Education, Science and Culture, Japan.
PY - 1992/8/14
Y1 - 1992/8/14
N2 - Cytochrome b558 is the only membrane component of the phagocyte O2- NADPH oxidase. The O2- production by the oxidase reconstituted in vitro with the crude membrane fraction is enhanced several-fold by addition of FAD, whereas that with the partially purified cytochrome is completely dependent on exogenous FAD, suggesting that FAD acts through the membrane component, cytochrome b558. The alignments of the amino acid sequence of the large subunit of the cytochrome (gp91-phox) with those of previously characterized flavoproteins reveal that the middle and C-terminal portions of gp91-phox are likely to be FAD- and NADPHbinding domains, respectively. Cytochrome b558, thus, appears to be a flavoprotein with an NADPH-binding site, of the NADPH oxidase.
AB - Cytochrome b558 is the only membrane component of the phagocyte O2- NADPH oxidase. The O2- production by the oxidase reconstituted in vitro with the crude membrane fraction is enhanced several-fold by addition of FAD, whereas that with the partially purified cytochrome is completely dependent on exogenous FAD, suggesting that FAD acts through the membrane component, cytochrome b558. The alignments of the amino acid sequence of the large subunit of the cytochrome (gp91-phox) with those of previously characterized flavoproteins reveal that the middle and C-terminal portions of gp91-phox are likely to be FAD- and NADPHbinding domains, respectively. Cytochrome b558, thus, appears to be a flavoprotein with an NADPH-binding site, of the NADPH oxidase.
UR - http://www.scopus.com/inward/record.url?scp=0027007734&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027007734&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(05)81557-8
DO - 10.1016/S0006-291X(05)81557-8
M3 - Article
C2 - 1324665
AN - SCOPUS:0027007734
SN - 0006-291X
VL - 186
SP - 1368
EP - 1375
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -