TY - JOUR
T1 - Cutting edge
T2 - Dominant effect of Ile50Val variant of the human IL-4 receptor α-chain in IgE synthesis
AU - Mitsuyasu, Hiromichi
AU - Yanagihara, Yukiyoshi
AU - Mao, Xiao Quan
AU - Gao, Pei Sun
AU - Arinobu, Yojiro
AU - Ihara, Kenji
AU - Takabayashi, Akira
AU - Hara, Toshiro
AU - Enomoto, Tadao
AU - Sasaki, Sei
AU - Kawai, Minoru
AU - Hamasaki, Naotaka
AU - Shirakawa, Taro
AU - Hopkin, Julian M.
AU - Izuhara, Kenji
PY - 1999/2/1
Y1 - 1999/2/1
N2 - Two variant of the IL-4R α-chain (IL-4Rα) gene have been recently identified in association with different atopic disorders. To clarify the etiological relationship between the two variants, we analyzed responsiveness to IL-4 of transfectants with four kinds of IL-4Rα carrying either Val or Ile at 50 and either Gln or Arg at 551. The substitution of Ile for Val augmented STAT6 activation, proliferation, and transcription activity of the Iε promoter by IL-4, whereas that of Arg for Gln did not change these IL-4 signals. Arg551 was not associated with atopic asthma in the Japanese population. CD23 expression and IgE synthesis by IL-4 were augmented in Ile50-bearing PBMC, compared with those bearing Val50. Taken together, substitution of Arg551 does not enhance the IL-4 signal for generation of germline ε transcript, whereas the substitution of Ile50 contributes to enhancement of IgE synthesis.
AB - Two variant of the IL-4R α-chain (IL-4Rα) gene have been recently identified in association with different atopic disorders. To clarify the etiological relationship between the two variants, we analyzed responsiveness to IL-4 of transfectants with four kinds of IL-4Rα carrying either Val or Ile at 50 and either Gln or Arg at 551. The substitution of Ile for Val augmented STAT6 activation, proliferation, and transcription activity of the Iε promoter by IL-4, whereas that of Arg for Gln did not change these IL-4 signals. Arg551 was not associated with atopic asthma in the Japanese population. CD23 expression and IgE synthesis by IL-4 were augmented in Ile50-bearing PBMC, compared with those bearing Val50. Taken together, substitution of Arg551 does not enhance the IL-4 signal for generation of germline ε transcript, whereas the substitution of Ile50 contributes to enhancement of IgE synthesis.
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M3 - Article
C2 - 9973373
AN - SCOPUS:0033083286
SN - 0022-1767
VL - 162
SP - 1227
EP - 1231
JO - Journal of Immunology
JF - Journal of Immunology
IS - 3
ER -