TY - JOUR
T1 - Crystallization and preliminary X-ray diffraction analysis of an active-site mutant of 'loopless' family GH19 chitinase from Bryum coronatum in a complex with chitotetraose
AU - Ohnuma, Takayuki
AU - Umemoto, Naoyuki
AU - Taira, Toki
AU - Fukamizo, Tamo
AU - Numata, Tomoyuki
PY - 2013/12
Y1 - 2013/12
N2 - The catalytic mechanism of family GH19 chitinases is not well understood owing to insufficient information regarding the three-dimensional structures of enzyme-substrate complexes. Here, the crystallization and preliminary X-ray diffraction analysis of a selenomethionine-labelled active-site mutant of 'loopless' family GH19 chitinase from the moss Bryum coronatum in complex with chitotetraose, (GlcNAc)4, are reported. The crystals were grown using the vapour-diffusion method. They diffracted to 1.58Å resolution using synchrotron radiation at the Photon Factory. The crystals belonged to the monoclinic space group C2, with unit-cell parameters a = 74.5, b = 58.4, c = 48.1Å, β = 115.6°. The asymmetric unit of the crystals is expected to contain one protein molecule, with a Matthews coefficient of 2.08Å3Da-1 and a solvent content of 41%.
AB - The catalytic mechanism of family GH19 chitinases is not well understood owing to insufficient information regarding the three-dimensional structures of enzyme-substrate complexes. Here, the crystallization and preliminary X-ray diffraction analysis of a selenomethionine-labelled active-site mutant of 'loopless' family GH19 chitinase from the moss Bryum coronatum in complex with chitotetraose, (GlcNAc)4, are reported. The crystals were grown using the vapour-diffusion method. They diffracted to 1.58Å resolution using synchrotron radiation at the Photon Factory. The crystals belonged to the monoclinic space group C2, with unit-cell parameters a = 74.5, b = 58.4, c = 48.1Å, β = 115.6°. The asymmetric unit of the crystals is expected to contain one protein molecule, with a Matthews coefficient of 2.08Å3Da-1 and a solvent content of 41%.
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U2 - 10.1107/S1744309113028935
DO - 10.1107/S1744309113028935
M3 - Article
C2 - 24316830
AN - SCOPUS:84890060798
SN - 1744-3091
VL - 69
SP - 1360
EP - 1362
JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
IS - 12
ER -