A new member of archaeal DNA polymerase from Pyrococcus furiosus was crystallized. Diffraction data to 3.1 Å of the selenomethionine- derivatized crystal were collected, and preliminary crystallographic study has been completed. The crystal belongs to the space group C2 with unit cell parameters of a = 93.2 Å, b = 124.9 Å, c = 87.7 Å, α = 90° , β = 109.7° , and γ = 90°. Assuming the presence of one molecule in the asymmetric unit, the solvent content of the crystal is estimated to be 54%, corresponding to a Matthews coefficient VM of 2.7Å3 Da-1.
All Science Journal Classification (ASJC) codes
- Structural Biology