TY - JOUR
T1 - Crystallization and preliminary crystallographic study of DNA polymerase from Pyrococcus furiosus
AU - Nishida, Hirokazu
AU - Tanabe, Maiko
AU - Ishino, Yoshizumi
AU - Oyama, Takuji
AU - Morikawa, Kosuke
PY - 2007/4
Y1 - 2007/4
N2 - A new member of archaeal DNA polymerase from Pyrococcus furiosus was crystallized. Diffraction data to 3.1 Å of the selenomethionine- derivatized crystal were collected, and preliminary crystallographic study has been completed. The crystal belongs to the space group C2 with unit cell parameters of a = 93.2 Å, b = 124.9 Å, c = 87.7 Å, α = 90° , β = 109.7° , and γ = 90°. Assuming the presence of one molecule in the asymmetric unit, the solvent content of the crystal is estimated to be 54%, corresponding to a Matthews coefficient VM of 2.7Å3 Da-1.
AB - A new member of archaeal DNA polymerase from Pyrococcus furiosus was crystallized. Diffraction data to 3.1 Å of the selenomethionine- derivatized crystal were collected, and preliminary crystallographic study has been completed. The crystal belongs to the space group C2 with unit cell parameters of a = 93.2 Å, b = 124.9 Å, c = 87.7 Å, α = 90° , β = 109.7° , and γ = 90°. Assuming the presence of one molecule in the asymmetric unit, the solvent content of the crystal is estimated to be 54%, corresponding to a Matthews coefficient VM of 2.7Å3 Da-1.
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U2 - 10.2174/092986607780363853
DO - 10.2174/092986607780363853
M3 - Article
C2 - 17504099
AN - SCOPUS:34147152912
SN - 0929-8665
VL - 14
SP - 403
EP - 405
JO - Protein and Peptide Letters
JF - Protein and Peptide Letters
IS - 4
ER -