TY - JOUR
T1 - Crystallization and preliminary crystallographic analysis of the N-terminal domain of PriA from Escherichia coli
AU - Sasaki, Kaori
AU - Ose, Toyoyuki
AU - Tanaka, Taku
AU - Mizukoshi, Toshimi
AU - Ishigaki, Tomoko
AU - Maenaka, Katsumi
AU - Masai, Hisao
AU - Kohda, Daisuke
N1 - Funding Information:
We would like to thank M. Kawamoto, H. Sakai, K. Hasegawa, N. Shimizu, and K. Miura for assistance with data collection at SPring-8, Harima, Japan. We also would like to thank S. Wakatsuki, N. Igarashi, and N. Matsugaki for kind advice during the data collection at Photon Factory, Tsukuba, Japan. KM and DK were supported by Grants-in-Aid for Scientific Research in Priority Areas and National Project on Protein Structural and Functional Analyses (Protein 3000) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan. KS was supported by research fellowship from Inoue Foundation for Science, and TO from Japan Society for the Promotion of Science.
PY - 2006/1
Y1 - 2006/1
N2 - PriA, a DEXH-type DNA helicase, binds specifically to the 3′ end of DNA through its N-terminal domain, and is a candidate sensor protein that recognizes arrested DNA replication forks in bacteria. We crystallized an N-terminal fragment of PriA in the absence and the presence of oligonucleotides to elucidate the structural basis for the specific recognition of the 3′ terminus of DNA.
AB - PriA, a DEXH-type DNA helicase, binds specifically to the 3′ end of DNA through its N-terminal domain, and is a candidate sensor protein that recognizes arrested DNA replication forks in bacteria. We crystallized an N-terminal fragment of PriA in the absence and the presence of oligonucleotides to elucidate the structural basis for the specific recognition of the 3′ terminus of DNA.
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U2 - 10.1016/j.bbapap.2005.09.007
DO - 10.1016/j.bbapap.2005.09.007
M3 - Article
C2 - 16226927
AN - SCOPUS:33644834103
SN - 1570-9639
VL - 1764
SP - 157
EP - 160
JO - Biochimica et Biophysica Acta - Proteins and Proteomics
JF - Biochimica et Biophysica Acta - Proteins and Proteomics
IS - 1
ER -