TY - JOUR
T1 - Conversion of short-chain ceramides to short-chain ceramide GM3 in B16 melanoma cells
AU - Komori, Hironobu
AU - Ito, Makoto
N1 - Funding Information:
Acknowledgements: This work was supported in part by a Grant-in-Aid for Scientific Research on Priority Areas (Nos. 05274107 and 06454657) from the Ministry of Education, Science and Culture of Japan, and the Mizutani Foundation for Glycoscience.
PY - 1995/10/30
Y1 - 1995/10/30
N2 - We report that short-chain ceramide (Cer), C2- and C6Cer, were immediately glycosylated and finally converted to short-chain Cer GM3 in B16 melanoma cells. By addition of either C2- or C6Cer to a cell culture of B16 melanoma in the presence of [14C]Gal, the radiolabeled precursor, was incorporated into each of two novel glycosphingolipids (GSLs) within 30 min along with synthesis of normal GSLs. These novel GSLs were identified as C2-, C6Cer cerebrosides and C2-, C6Cer GM3, respectively. In comparison with C2Cer, C6Cer was found to be much more efficiently converted to the GSLs, whereas no glycosylated sphingosine was detectable when it was added in place of short-chain Cer.
AB - We report that short-chain ceramide (Cer), C2- and C6Cer, were immediately glycosylated and finally converted to short-chain Cer GM3 in B16 melanoma cells. By addition of either C2- or C6Cer to a cell culture of B16 melanoma in the presence of [14C]Gal, the radiolabeled precursor, was incorporated into each of two novel glycosphingolipids (GSLs) within 30 min along with synthesis of normal GSLs. These novel GSLs were identified as C2-, C6Cer cerebrosides and C2-, C6Cer GM3, respectively. In comparison with C2Cer, C6Cer was found to be much more efficiently converted to the GSLs, whereas no glycosylated sphingosine was detectable when it was added in place of short-chain Cer.
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U2 - 10.1016/0014-5793(95)01137-4
DO - 10.1016/0014-5793(95)01137-4
M3 - Article
C2 - 7589558
AN - SCOPUS:0028880681
SN - 0014-5793
VL - 374
SP - 299
EP - 302
JO - FEBS Letters
JF - FEBS Letters
IS - 2
ER -