Control of vascular endothelial cell function by changing the pore size of self-organized porous films

Emiko Itoh; Aiko Takayama; Masaru Tanaka; Sadaaki Yamamoto; Masatsugu Simomura

Control of vascular endothelial cell function by changing the pore size of self-organized porous films

Emiko Itoh, Aiko Takayama, Masaru Tanaka, Sadaaki Yamamoto, Masatsugu Simomura

Research output: Contribution to conference › Paper › peer-review

Abstract

Self-organized porous films (honeycomb films) with different pore size were prepared in order to investigate the influence of pore size on porcine arterial endothelial cells (PAECs) behavior. These films were fabricated by applying a moist air to a spread polymer solution containing biodegradable polymers (poly (ε-caprolactone)(PCL)) and an amphiphilic polymer. Four different pore sizes (5,10,12,16 μm) were prepared. Changes in cell morphology, proliferation, cytoskeleton and extra cellular matrix (ECM) (fibronectin, laminin, type IV collagen, elastin) production profiles were observed by optical, fluorescence, and scanning electron microscopy. The degree of spreading of PAECs was enhanced with increase of the pore size. PAECs proliferated on the honeycomb films. Almost the same ECM production profiles were obtained on the honeycomb films. These suggest that the honeycomb film is suitable as a material of a new vascular device.

Original language	English
Pages	2234
Number of pages	1
Publication status	Published - 2005
Externally published	Yes
Event	54th SPSJ Annual Meeting 2005 - Yokohama, Japan Duration: May 25 2005 → May 27 2005

Other

Other	54th SPSJ Annual Meeting 2005
Country/Territory	Japan
City	Yokohama
Period	5/25/05 → 5/27/05

All Science Journal Classification (ASJC) codes

Engineering(all)

Cite this

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title = "Control of vascular endothelial cell function by changing the pore size of self-organized porous films",

abstract = "Self-organized porous films (honeycomb films) with different pore size were prepared in order to investigate the influence of pore size on porcine arterial endothelial cells (PAECs) behavior. These films were fabricated by applying a moist air to a spread polymer solution containing biodegradable polymers (poly (ε-caprolactone)(PCL)) and an amphiphilic polymer. Four different pore sizes (5,10,12,16 μm) were prepared. Changes in cell morphology, proliferation, cytoskeleton and extra cellular matrix (ECM) (fibronectin, laminin, type IV collagen, elastin) production profiles were observed by optical, fluorescence, and scanning electron microscopy. The degree of spreading of PAECs was enhanced with increase of the pore size. PAECs proliferated on the honeycomb films. Almost the same ECM production profiles were obtained on the honeycomb films. These suggest that the honeycomb film is suitable as a material of a new vascular device.",

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year = "2005",

language = "English",

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note = "54th SPSJ Annual Meeting 2005 ; Conference date: 25-05-2005 Through 27-05-2005",

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TY - CONF

T1 - Control of vascular endothelial cell function by changing the pore size of self-organized porous films

AU - Itoh, Emiko

AU - Takayama, Aiko

AU - Tanaka, Masaru

AU - Yamamoto, Sadaaki

AU - Simomura, Masatsugu

PY - 2005

Y1 - 2005

N2 - Self-organized porous films (honeycomb films) with different pore size were prepared in order to investigate the influence of pore size on porcine arterial endothelial cells (PAECs) behavior. These films were fabricated by applying a moist air to a spread polymer solution containing biodegradable polymers (poly (ε-caprolactone)(PCL)) and an amphiphilic polymer. Four different pore sizes (5,10,12,16 μm) were prepared. Changes in cell morphology, proliferation, cytoskeleton and extra cellular matrix (ECM) (fibronectin, laminin, type IV collagen, elastin) production profiles were observed by optical, fluorescence, and scanning electron microscopy. The degree of spreading of PAECs was enhanced with increase of the pore size. PAECs proliferated on the honeycomb films. Almost the same ECM production profiles were obtained on the honeycomb films. These suggest that the honeycomb film is suitable as a material of a new vascular device.

AB - Self-organized porous films (honeycomb films) with different pore size were prepared in order to investigate the influence of pore size on porcine arterial endothelial cells (PAECs) behavior. These films were fabricated by applying a moist air to a spread polymer solution containing biodegradable polymers (poly (ε-caprolactone)(PCL)) and an amphiphilic polymer. Four different pore sizes (5,10,12,16 μm) were prepared. Changes in cell morphology, proliferation, cytoskeleton and extra cellular matrix (ECM) (fibronectin, laminin, type IV collagen, elastin) production profiles were observed by optical, fluorescence, and scanning electron microscopy. The degree of spreading of PAECs was enhanced with increase of the pore size. PAECs proliferated on the honeycomb films. Almost the same ECM production profiles were obtained on the honeycomb films. These suggest that the honeycomb film is suitable as a material of a new vascular device.

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M3 - Paper

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T2 - 54th SPSJ Annual Meeting 2005

Y2 - 25 May 2005 through 27 May 2005

ER -

Control of vascular endothelial cell function by changing the pore size of self-organized porous films

Abstract

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