TY - JOUR
T1 - Control of neural stem cell differentiation on honeycomb films
AU - Tsuruma, Akinori
AU - Tanaka, Masaru
AU - Yamamoto, Sadaaki
AU - Shimomura, Masatsugu
N1 - Funding Information:
This study is supported by grants-in-Aid from Japan Science and Technology Corporation (JST) and special coordination funds for promotion science and technology.
PY - 2008/2/1
Y1 - 2008/2/1
N2 - Control of neural stem cell (NSC) differentiation is ongoing interest in neural tissue engineering. Formation of neural networks on various patterned substrates was reported in previous studies. In this study, we cultured NSCs derived from the cerebral cortex of embryonic day-14 mice on honeycomb (HC) films with highly regular pores prepared by casting a polymer solution of water-immiscible solvent under high humidity. The differentiation of NSCs was analyzed by immunostaining for Nestin and MAP2. The differentiation of NSC was controlled for the first time by manipulating the pore size on HC films. The highest suppression of NSC differentiation was observed on HC film with 3 μm pore specifically.
AB - Control of neural stem cell (NSC) differentiation is ongoing interest in neural tissue engineering. Formation of neural networks on various patterned substrates was reported in previous studies. In this study, we cultured NSCs derived from the cerebral cortex of embryonic day-14 mice on honeycomb (HC) films with highly regular pores prepared by casting a polymer solution of water-immiscible solvent under high humidity. The differentiation of NSCs was analyzed by immunostaining for Nestin and MAP2. The differentiation of NSC was controlled for the first time by manipulating the pore size on HC films. The highest suppression of NSC differentiation was observed on HC film with 3 μm pore specifically.
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U2 - 10.1016/j.colsurfa.2007.05.079
DO - 10.1016/j.colsurfa.2007.05.079
M3 - Article
AN - SCOPUS:37349026184
SN - 0927-7757
VL - 313-314
SP - 536
EP - 540
JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects
JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects
ER -