Control of mass transport properties of cell embedding materials

The gels using cell embedding is require of the profiles of bio- compatibility and selective transport properties. In usually, it is difficult that we build one materials of having the both properties. In this works, we tried preparation of the laminated gel of agarose gel and acrylamide gel. Agarose gel have a promotion of bio-compatibility, because this gel is not decomposed by enzyme in vivo. Polyacrylamide gel is easy control mass transport properties. The mass transport properties as gel evaluated from the diffusion coefficient of probe molecular. Probe moleculars are used glucose, vitamin B ₁₂, and bovine serum albumin. The diffusion coefficients of probe molecules in gel were determined by the time-lag technique. The laminated gel of agarose gel and polyacrylamide gel is blocked the permeation of macromolecular. Further, the permeation of low molecular throw the laminated gel was similar as that of agarose gel. These results is suggestion the impossible to create the cell embedding gel as the combine to both gel at biocompatibility and selective permeability. These informations obtained by the transport experiments are necessary to design the bio-interface materials.