TY - JOUR
T1 - Control of biomimetic hydroxyapatite deposition on polymer substrates using different protein adsorption abilities
AU - Iijima, Kazutoshi
AU - Sakai, Atsushi
AU - Komori, Akinori
AU - Sakamoto, Yuri
AU - Matsuno, Hisao
AU - Serizawa, Takeshi
AU - Hashizume, Mineo
N1 - Funding Information:
The authors thank Prof. T. Kawai (Tokyo University of Science) for the use of the vapor deposition apparatus. This work was partly supported by Grants-in-Aid for Young Scientists (B) from MEXT, Japan (M. H., 20710088 ).
Publisher Copyright:
© 2015 Elsevier B.V.
PY - 2015/6/1
Y1 - 2015/6/1
N2 - We recently developed a system for coating polystyrene (PS) substrates with hydroxyapatite (HAp) by utilizing serum protein adsorption layers as mediators to induce the heterogeneous nucleation of HAp in simulated body fluids (SBFs). In this study, the selective deposition of HAp on polymer substrate surfaces with different protein adsorption abilities was investigated using PS and poly(methyl methacrylate) (PMMA). Atomic force microscopic observations and the results of a quantitative analysis using a quartz-crystal microbalance (QCM) revealed that the amounts of proteins such as human serum albumin (HSA) and human immunoglobulin G (hIgG) adsorbed on PS substrate surfaces were markedly greater than those on PMMA substrate surfaces. A markedly larger amount of HAp was deposited on protein-treated PS substrate surfaces than on PMMA substrate surfaces, reflecting protein adsorption to polymers. We also revealed that the deposition of HAp on protein-adsorbed PS substrate surfaces was enhanced by aqueous calcium chloride treatments before immersion in 1.5SBF. In the case of 2.5. M calcium chloride treatment, these surfaces were completely covered with deposits.
AB - We recently developed a system for coating polystyrene (PS) substrates with hydroxyapatite (HAp) by utilizing serum protein adsorption layers as mediators to induce the heterogeneous nucleation of HAp in simulated body fluids (SBFs). In this study, the selective deposition of HAp on polymer substrate surfaces with different protein adsorption abilities was investigated using PS and poly(methyl methacrylate) (PMMA). Atomic force microscopic observations and the results of a quantitative analysis using a quartz-crystal microbalance (QCM) revealed that the amounts of proteins such as human serum albumin (HSA) and human immunoglobulin G (hIgG) adsorbed on PS substrate surfaces were markedly greater than those on PMMA substrate surfaces. A markedly larger amount of HAp was deposited on protein-treated PS substrate surfaces than on PMMA substrate surfaces, reflecting protein adsorption to polymers. We also revealed that the deposition of HAp on protein-adsorbed PS substrate surfaces was enhanced by aqueous calcium chloride treatments before immersion in 1.5SBF. In the case of 2.5. M calcium chloride treatment, these surfaces were completely covered with deposits.
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U2 - 10.1016/j.colsurfb.2015.04.010
DO - 10.1016/j.colsurfb.2015.04.010
M3 - Article
C2 - 25909182
AN - SCOPUS:84928139549
SN - 0927-7765
VL - 130
SP - 77
EP - 83
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
ER -