Contrast-tuneable microscopy for single-shot real-time imaging

Isao Shimizu, Yoshinori Saikawa, Katsuhiro Uno, Hideaki Kano, Seishi Shimizu

Research output: Contribution to journalArticlepeer-review


A novel real image in-line laser holography has enabled a tuneable image contrast, edge sharpness, and visualization of sub-wavelength structures, using a simple pair of filters and large-diameter lenses that can incorporate higher-order scattered beams. Demonstrated also are the accuracy in object sizing and the ease of imaging along the focal depth, based on a single-shot imaging via holographic principle. In addition, the use of broad, collimated laser beam for irradiation has led to a wider field of view, making it particularly useful for an extensive monitoring of, and sweeping search for, cells and microbial colonies and for the real-time imaging of cancer-cell dynamics.

Original languageEnglish
Article number30701
JournalEPJ Applied Physics
Issue number3
Publication statusPublished - Sept 1 2020
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics


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