Continuous production of human erythropoietin by immobilized recombinant L-929 cells

Shuji Terashima, Masamichi Kamihira, Tatsuya Ogawa, Masanao Ohno, Shinji Iijima, Takeshi Kobayashi

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16 Citations (Scopus)


Recombinant L-929 cells transfected with the human erythropoietin (EPO) gene were immobilized in a macroporous cellulosic support and its derivatives in which charged groups or cell attachment factors were introduced. The immobilized cells were cultured in serum-containing and serum-free media. Comparable production of EPO was observed even in the serum-free medium when a support modified by polyethyleneimine was used for immobilization. The cells immobilized on the supports were cultured in fluidized-bed and inner-loop type air-lift bioreactors for continuous production of EPO. A high cell density of more than 2 × 107 cells/cm3-support and high EPO productivity were achieved and maintained for 50 d through the use of the inner-loop type air-lift bioreactor. The productivity was 13.4-fold higher than that of conventional static cultures in petri-dishes.

Original languageEnglish
Pages (from-to)52-56
Number of pages5
JournalJournal of Fermentation and Bioengineering
Issue number1
Publication statusPublished - 1994
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology


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