Construction of the single-chain Fv from 196-14 antibody toward ovarian cancer-associated antigen CA125

Yoshio Hashimoto; Kiyoshi Tanigawa; Manabu Nakashima; Kenzo Sonoda; Tadashi Ueda; Takeshi Watanabe; Taiji Imoto

doi:10.1248/bpb.22.1068

Construction of the single-chain Fv from 196-14 antibody toward ovarian cancer-associated antigen CA125

Yoshio Hashimoto, Kiyoshi Tanigawa, Manabu Nakashima, Kenzo Sonoda, Tadashi Ueda, Takeshi Watanabe, Taiji Imoto

Reproductive and Developmental Medicine

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

The variable regions of heavy- and light-chains of mouse monoclonal antibody 196-14 toward ovarian cancer-associated antigen CA125 were linked with a peptide linker (GSTSGSGKSSEGKG) and a histidine tag was attached at the carboxyl terminal. This single-chain Fv (scFv) with a histidine tag was expressed in Escherichia coli as an inclusion body. The inclusion body was solubilized with guanidium chloride, followed by purification on nickel nitrilotriacetic acid agarose column and refolding into the active form. The scFv thus obtained bound to the Siso cells, which express CA125, and may recognize the same epitope as the parental 196-14 antibody IgG does.

Original language	English
Pages (from-to)	1068-1072
Number of pages	5
Journal	Biological and Pharmaceutical Bulletin
Volume	22
Issue number	10
DOIs	https://doi.org/10.1248/bpb.22.1068
Publication status	Published - Oct 1999

All Science Journal Classification (ASJC) codes

Pharmacology
Pharmaceutical Science

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T1 - Construction of the single-chain Fv from 196-14 antibody toward ovarian cancer-associated antigen CA125

AU - Hashimoto, Yoshio

AU - Tanigawa, Kiyoshi

AU - Nakashima, Manabu

AU - Sonoda, Kenzo

AU - Ueda, Tadashi

AU - Watanabe, Takeshi

AU - Imoto, Taiji

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Construction of the single-chain Fv from 196-14 antibody toward ovarian cancer-associated antigen CA125

Abstract

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