TY - GEN
T1 - Conformation and dynamic behavior of single DNA molecules in nanofluidic channels for detection of DNA methylation
AU - Sun, X.
AU - Yasui, T.
AU - Rahong, S.
AU - Yanagida, T.
AU - Kaji, N.
AU - Kanai, M.
AU - Nagashima, K.
AU - Kawai, T.
AU - Baba, Y.
N1 - Funding Information:
This work was partly supported by the Nanotechnology Platform Program (Molecule and Material Synthesis) of the Ministry of Education, Culture, Sports, Science and Technology (MEXT), the JSPS Grant-in-Aid for Scientific Research (A) 24241050.
Publisher Copyright:
© 15CBMS-0001.
PY - 2015
Y1 - 2015
N2 - In this paper, the single DNA molecules were linearized and stretched by using the nanochannel with a dimension of about 300 × 300 nm2 under applying electric fields. After that, the single DNA molecules methylation level was detected by estimating the contraction processes of both non-methylated and methylated single DNA molecules in nanochannel after switching off the electric fields. And furthermore, we also found that their translocation velocities were changed between non-methylated and methylated single DNA molecules in nanochannel.
AB - In this paper, the single DNA molecules were linearized and stretched by using the nanochannel with a dimension of about 300 × 300 nm2 under applying electric fields. After that, the single DNA molecules methylation level was detected by estimating the contraction processes of both non-methylated and methylated single DNA molecules in nanochannel after switching off the electric fields. And furthermore, we also found that their translocation velocities were changed between non-methylated and methylated single DNA molecules in nanochannel.
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M3 - Conference contribution
AN - SCOPUS:84983350434
T3 - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 1115
EP - 1117
BT - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
Y2 - 25 October 2015 through 29 October 2015
ER -