TY - JOUR
T1 - Computed tomography for in vivo deep over-1000 nm near-infrared fluorescence imaging
AU - Umezawa, Masakazu
AU - Sera, Toshihiro
AU - Yokota, Hideo
AU - Takematsu, Maho
AU - Morita, Masahiko
AU - Yeroslavsky, Gil
AU - Kamimura, Masao
AU - Soga, Kohei
N1 - Funding Information:
We thank the graduate and undergraduate students for their technical assistance in the Soga Laboratory in Tokyo University of Science, especially Mr. Soichiro Fujii and Mr. Takeaki Harada for optical system setup, Mr. Ryuta Takeda and Mr. Takaya Yamada for preparation of NaGdF co‐doped with Yb and Ho, and Ms. Yuki Uenomachi for optimizing agarose gel‐based biological phantom. This work was supported in part by the MEXT Grant‐in‐Aid for Scientific Research on Innovative Areas (Resonance Bio), no. 15H05950, the MEXT Grant‐in‐Aid for Scientific Research (A), no. 19H01179, the MEXT‐Supported Program for the Strategic Research Foundation at Private Universities, no. S1511012, and the Center of Innovation Program “COINS” from Japan Science and Technology Agency, JST. 4 3+ 3+
Funding Information:
Ministry of Education, Culture, Sports, Science and Technology, Grant/Award Numbers: S1511012, 19H01179, 15H05950; Japan Science and Technology Agency, Grant/Award Number: Center of Innovation Program “COINS” Funding information
Funding Information:
We thank the graduate and undergraduate students for their technical assistance in the Soga Laboratory in Tokyo University of Science, especially Mr. Soichiro Fujii and Mr. Takeaki Harada for optical system setup, Mr. Ryuta Takeda and Mr. Takaya Yamada for preparation of NaGdF4 co-doped with Yb3+ and Ho3+, and Ms. Yuki Uenomachi for optimizing agarose gel-based biological phantom. This work was supported in part by the MEXT Grant-in-Aid for Scientific Research on Innovative Areas (Resonance Bio), no. 15H05950, the MEXT Grant-in-Aid for Scientific Research (A), no. 19H01179, the MEXT-Supported Program for the Strategic Research Foundation at Private Universities, no. S1511012, and the Center of Innovation Program ?COINS? from Japan Science and Technology Agency, JST.
Publisher Copyright:
© 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2020/8/1
Y1 - 2020/8/1
N2 - This study aims to develop a novel cross-sectional imaging of fluorescence in over-1000 nm near-infrared (OTN-NIR), which allows in vivo deep imaging, using computed tomography (CT) system. Cylindrical specimens of composite of OTN-NIR fluorophore, NaGdF4 co-doped with Yb3+ and Ho3+ (ex: 980 nm, em: 1150 nm), were embedded in cubic agar (10.5–12 mm) or in the peritoneal cavity of mice and placed on a rotatable stage. When the fluorescence from inside of the samples was serially captured from multiple angles, the images were disrupted by the reflection and refraction of emitted light on the sample-air interface. Immersing the sample into water filled in a rectangular bath suppressed the disruption at the interface and successfully reconstructed the position and concentration of OTN-NIR fluorophores on the cross-sectional images using a CT technique. This is promising as a novel three-dimensional imaging technique for OTN-NIR fluorescent image projections of small animals captured from multiple angles.
AB - This study aims to develop a novel cross-sectional imaging of fluorescence in over-1000 nm near-infrared (OTN-NIR), which allows in vivo deep imaging, using computed tomography (CT) system. Cylindrical specimens of composite of OTN-NIR fluorophore, NaGdF4 co-doped with Yb3+ and Ho3+ (ex: 980 nm, em: 1150 nm), were embedded in cubic agar (10.5–12 mm) or in the peritoneal cavity of mice and placed on a rotatable stage. When the fluorescence from inside of the samples was serially captured from multiple angles, the images were disrupted by the reflection and refraction of emitted light on the sample-air interface. Immersing the sample into water filled in a rectangular bath suppressed the disruption at the interface and successfully reconstructed the position and concentration of OTN-NIR fluorophores on the cross-sectional images using a CT technique. This is promising as a novel three-dimensional imaging technique for OTN-NIR fluorescent image projections of small animals captured from multiple angles.
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U2 - 10.1002/jbio.202000071
DO - 10.1002/jbio.202000071
M3 - Article
C2 - 32388908
AN - SCOPUS:85086036497
SN - 1864-063X
VL - 13
JO - Journal of Biophotonics
JF - Journal of Biophotonics
IS - 8
M1 - e202000071
ER -