Comprehensive identification of substrates for f-box proteins by differential proteomics analysis

Kanae Yumimoto, Masaki Matsumoto, Koji Oyamada, Toshiro Moroishi, Keiichi I. Nakayama

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)


Although elucidation of enzyme-substrate relations is fundamental to the advancement of biology, universal approaches to the identification of substrates for a given enzyme have not been established. It is especially difficult to identify substrates for ubiquitin ligases, given that most such substrates are immediately ubiquitylated and degraded as a result of their association with the enzyme. We here describe the development of a new approach, DiPIUS (differential proteomics-based identification of ubiquitylation substrates), to the discovery of substrates for ubiquitin ligases. We applied DiPIUS to Fbxw7α, Skp2, and Fbxl5, three of the most well-characterized F-box proteins, and identified candidate substrates including previously known targets. DiPIUS is thus a powerful tool for unbiased and comprehensive screening for substrates of ubiquitin ligases.

Original languageEnglish
Pages (from-to)3175-3185
Number of pages11
JournalJournal of Proteome Research
Issue number6
Publication statusPublished - Jun 1 2012

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • General Chemistry


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