TY - JOUR
T1 - Comprehensive analysis of glucan elicitor-regulated gene expression in tobacco BY-2 cells reveals a novel MYB transcription factor involved in the regulation of phenylpropanoid metabolism
AU - Shinya, Tomonori
AU - Gális, Ivan
AU - Narisawa, Tomoko
AU - Sasaki, Mami
AU - Fukuda, Hiroo
AU - Matsuoka, Hideaki
AU - Saito, Mikako
AU - Matsuoka, Ken
N1 - Funding Information:
This work was supported in part by a Grant-in-aid for Scientific Research of Priority Areas: Single-Cell Molecular Technology (No. 11227101), from the Ministry of Education, Culture, Sports, Science and Technology to H.M.
PY - 2007/10
Y1 - 2007/10
N2 - We previously demonstrated that a β-1,3-, 1,6-oligoglucan (AaGlucan) from the fungus Alternaria alternata 102 shows strong elicitor activity in tobacco BY-2 cells. We have used cDNA microarray analysis to monitor global changes in gene expression in tobacco cells treated with this A. alternata fraction or with laminarin. In total, we identified 265 genes that were induced 1 h after treatment with an AaGlucan-enriched fraction or laminarin. Among them, we characterized in detail a novel tobacco R2R3 MYB-type transcription factor homolog (NtMYBGR1) and two DC1 domain-containing genes (NtDC1A and NtDC1B). Microarray data, together with overexpression and metabolic analyses, indicated that NtMYBGR1, but not the NtDC1 proteins, primarily targets the phenylpropanoid synthesis-related genes PAL and 4CL. These results suggest that NtMYBGR1 specifically regulates defense responses in BY-2 cells by enhancing phenylpropanoid metabolism in response to AaGlucan and laminarin elicitors.
AB - We previously demonstrated that a β-1,3-, 1,6-oligoglucan (AaGlucan) from the fungus Alternaria alternata 102 shows strong elicitor activity in tobacco BY-2 cells. We have used cDNA microarray analysis to monitor global changes in gene expression in tobacco cells treated with this A. alternata fraction or with laminarin. In total, we identified 265 genes that were induced 1 h after treatment with an AaGlucan-enriched fraction or laminarin. Among them, we characterized in detail a novel tobacco R2R3 MYB-type transcription factor homolog (NtMYBGR1) and two DC1 domain-containing genes (NtDC1A and NtDC1B). Microarray data, together with overexpression and metabolic analyses, indicated that NtMYBGR1, but not the NtDC1 proteins, primarily targets the phenylpropanoid synthesis-related genes PAL and 4CL. These results suggest that NtMYBGR1 specifically regulates defense responses in BY-2 cells by enhancing phenylpropanoid metabolism in response to AaGlucan and laminarin elicitors.
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U2 - 10.1093/pcp/pcm115
DO - 10.1093/pcp/pcm115
M3 - Article
C2 - 17761750
AN - SCOPUS:35348901404
SN - 0032-0781
VL - 48
SP - 1404
EP - 1413
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
IS - 10
ER -