Complete sequence-specific ¹H nuclear magnetic resonance assignments for mouse epidermal growth factor

The ¹H NMR spectrum of the mouse epidermal growth factor (53 residues) was analyzed with the use of two-dimensional NMR techniques. All the observable 296 proton resonances were completely assigned in a sequential manner. For the spin system identification, two-dimensional homonuclear Hartmann-Hahn spectrum was useful, especially for arginine and proline residues. The easy spin system identification of these long-side-chainbearing amino acid residues greatly facilitated the sequence-specific resonance assignment of the epidermal growth factor.