TY - JOUR
T1 - Complete nucleotide sequence of pLD-TEX-KL, a 66-kb plasmid of Legionella dumoffii TEX-KL strain
AU - Qin, Tian
AU - Hirakawa, Hideki
AU - Iida, Ken ichiro
AU - Oshima, Kenshiro
AU - Hattori, Masahira
AU - Tashiro, Kosuke
AU - Kuhara, Satoru
AU - Yoshida, Shin ichi
N1 - Funding Information:
We thank Hiroaki Nakayama for scientific discussion. We also thank Hiromi Inaba, Yasue Yamashita, Noriko Ito, Asako Tamura, Keiko Furuya, Kanako Motomura and Chie Yoshino for technical assistances. This research was supported by Health and Labour Sciences research grants (H-16-Kenkou-055) from the Ministry of Health, Labour and Welfare, and a Grant-in-Aid for Scientific Research on Priority Areas “Comprehensive Genomics” from the Ministry of Education, Culture, Sports, Science and Technology of Japan (M.H.).
PY - 2007/11
Y1 - 2007/11
N2 - The complete nucleotide sequence of a large (66 kb) plasmid pLD-TEX-KL of Legionella dumoffii TEX-KL strain was determined. Of the 57 predicted open reading frames (ORFs), 39 (68%) encoded proteins similar to previously known proteins, five (9%) were assigned with putative functions, three (5%) encoded conserved hypothetical proteins, and 10 (18%) had no homology to any genes present in the current open databases. The ORFs with similar functions were organized in a modular structure; thus, transfer region was identified, as well as a putative heavy-metal ion transporter system (hel). The transfer region encoded homologs of the Salmonella entrica serovar Typhi conjugative system components involved in conjugation. In addition, we also found a potential protein that was analogous to the DNA polymerase III epsilon subunit. It is rarely found that plasmid encode the DNA polymerase.
AB - The complete nucleotide sequence of a large (66 kb) plasmid pLD-TEX-KL of Legionella dumoffii TEX-KL strain was determined. Of the 57 predicted open reading frames (ORFs), 39 (68%) encoded proteins similar to previously known proteins, five (9%) were assigned with putative functions, three (5%) encoded conserved hypothetical proteins, and 10 (18%) had no homology to any genes present in the current open databases. The ORFs with similar functions were organized in a modular structure; thus, transfer region was identified, as well as a putative heavy-metal ion transporter system (hel). The transfer region encoded homologs of the Salmonella entrica serovar Typhi conjugative system components involved in conjugation. In addition, we also found a potential protein that was analogous to the DNA polymerase III epsilon subunit. It is rarely found that plasmid encode the DNA polymerase.
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U2 - 10.1016/j.plasmid.2007.08.001
DO - 10.1016/j.plasmid.2007.08.001
M3 - Article
C2 - 17881053
AN - SCOPUS:35348955457
SN - 0147-619X
VL - 58
SP - 261
EP - 268
JO - Plasmid
JF - Plasmid
IS - 3
ER -