TY - JOUR
T1 - Complete nucleotide sequence of plasmid Rts1
T2 - Implications for evolution of large plasmid genomes
AU - Murata, Takahiro
AU - Ohnishi, Makoto
AU - Ara, Takeshi
AU - Kaneko, Jun
AU - Han, Chang Gyun
AU - Li, Yong Fang
AU - Takashima, Kayoko
AU - Nojima, Hideaki
AU - Nakayama, Keisuke
AU - Kaji, Akira
AU - Kamio, Yoshiyuki
AU - Miki, Takeyoshi
AU - Mori, Hirotada
AU - Ohtsubo, Eiichi
AU - Terawaki, Yoshiro
AU - Hayashi, Tetsuya
PY - 2002
Y1 - 2002
N2 - Rts1, a large conjugative plasmid originally isolated from Proteus vulgaris, is a prototype for the IncT plasmids and exhibits pleiotropic thermosensitive phenotypes. Here we report the complete nucleotide sequence of Rts1. The genome is 217,182 bp in length and contains 300 potential open reading frames (ORFs). Among these, the products of 141 ORFs, including 9 previously identified genes, displayed significant sequence similarity to known proteins. The set of genes responsible for the conjugation function of Rts1 has been identified. A broad array of genes related to diverse processes of DNA metabolism were also identified. Of particular interest was the presence of tus-like genes that could be involved in replication termination. Inspection of the overall genome organization revealed that the Rts1 genome is composed of four large modules, providing an example of modular evolution of plasmid genomes.
AB - Rts1, a large conjugative plasmid originally isolated from Proteus vulgaris, is a prototype for the IncT plasmids and exhibits pleiotropic thermosensitive phenotypes. Here we report the complete nucleotide sequence of Rts1. The genome is 217,182 bp in length and contains 300 potential open reading frames (ORFs). Among these, the products of 141 ORFs, including 9 previously identified genes, displayed significant sequence similarity to known proteins. The set of genes responsible for the conjugation function of Rts1 has been identified. A broad array of genes related to diverse processes of DNA metabolism were also identified. Of particular interest was the presence of tus-like genes that could be involved in replication termination. Inspection of the overall genome organization revealed that the Rts1 genome is composed of four large modules, providing an example of modular evolution of plasmid genomes.
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U2 - 10.1128/JB.184.12.3194-3202.2002
DO - 10.1128/JB.184.12.3194-3202.2002
M3 - Article
C2 - 12029035
AN - SCOPUS:0036267349
SN - 0021-9193
VL - 184
SP - 3194
EP - 3202
JO - Journal of bacteriology
JF - Journal of bacteriology
IS - 12
ER -