TY - JOUR
T1 - Complementary DNA for the mouse homolog of the small subunit of human cytochrome b558
AU - Sumimoto, Hideki
AU - Nozaki, Masahiko
AU - Sasaki, Hiroyuki
AU - Takeshige, Koichiro
AU - Sakaki, Yoshiyuki
AU - Minakami, Shigeki
N1 - Funding Information:
Acknowledgments: We would like to thank Dr. Youichiro Noguchi for instruction in Northern blot analysis and Ms. Hideko Ohgusu for preparing oligonucleotides. This work was supported in part by the Ministry of Education, Science and Culture, Japan.
PY - 1989/12/15
Y1 - 1989/12/15
N2 - Cytochrome b558, a heterodimeric protein, is considered to be a component of the microbicidal oxidase in phagocytes. We have isolated a cDNA clone for the mouse homolog of the small subunit of human cytochrome b558. Comparison of the predicted amino acid sequence with that of the human protein reveals a high degree of homology (87%). As expected, the mRNA is expressed abundantly in peritoneal neutrophils and, to a lesser extent, in peritoneal macrophages. The transcript is also abundant in the mouse kidney. Although the spleen and small intestine contain considerable amounts of the mRNA, only trace amounts are detected in the brain, liver, testis and heart. The distribution, especially its high expression in the kidney, may suggest another function of the small subunit.
AB - Cytochrome b558, a heterodimeric protein, is considered to be a component of the microbicidal oxidase in phagocytes. We have isolated a cDNA clone for the mouse homolog of the small subunit of human cytochrome b558. Comparison of the predicted amino acid sequence with that of the human protein reveals a high degree of homology (87%). As expected, the mRNA is expressed abundantly in peritoneal neutrophils and, to a lesser extent, in peritoneal macrophages. The transcript is also abundant in the mouse kidney. Although the spleen and small intestine contain considerable amounts of the mRNA, only trace amounts are detected in the brain, liver, testis and heart. The distribution, especially its high expression in the kidney, may suggest another function of the small subunit.
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U2 - 10.1016/S0006-291X(89)80051-8
DO - 10.1016/S0006-291X(89)80051-8
M3 - Article
C2 - 2597164
AN - SCOPUS:0024828829
SN - 0006-291X
VL - 165
SP - 902
EP - 906
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -