Competition between polysaccharide/polynucleotide complexation vs.polynucleotide hybridization; Salt concentration dependence of the reaction direction

Ryouji Karinaga; Kazuya Koumoto; Jusaku Minari; Masami Mizu; Takahisa Anada; Seiji Shinkai; Kazuo Sakurai

Competition between polysaccharide/polynucleotide complexation vs.polynucleotide hybridization; Salt concentration dependence of the reaction direction

Ryouji Karinaga
, Kazuya Koumoto
, Jusaku Minari
, Masami Mizu
, Takahisa Anada
, Seiji Shinkai
, Kazuo Sakurai

Research output: Contribution to conference › Paper › peer-review

Abstract

A single chain of schizophyllan (β-(1→3)-D-glucan) formed a complex with a homo-polynucleotide. In this paper, using adenine-thymine (or uracil) double strand as a model system, we explored whether s-SPG can unzip polynucleotide duplexes, examining the competition between the schizophyllan/polynucleotide complexation and the polynucleotide hybridization with circular dichroism at various NaCl concentrations. The unzipping reaction happened for the hetero-duplexes (i.e., RNA-DNA duplexes) at low salt concentrations, on the other hand, it was not observed for the homo-duplexes at all the salt concentrations.

Original language	English
Pages	2309
Number of pages	1
Publication status	Published - 2005
Externally published	Yes
Event	54th SPSJ Annual Meeting 2005 - Yokohama, Japan Duration: May 25 2005 → May 27 2005

Other

Other	54th SPSJ Annual Meeting 2005
Country/Territory	Japan
City	Yokohama
Period	5/25/05 → 5/27/05

All Science Journal Classification (ASJC) codes

General Engineering

Cite this

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title = "Competition between polysaccharide/polynucleotide complexation vs.polynucleotide hybridization; Salt concentration dependence of the reaction direction",

abstract = "A single chain of schizophyllan (β-(1→3)-D-glucan) formed a complex with a homo-polynucleotide. In this paper, using adenine-thymine (or uracil) double strand as a model system, we explored whether s-SPG can unzip polynucleotide duplexes, examining the competition between the schizophyllan/polynucleotide complexation and the polynucleotide hybridization with circular dichroism at various NaCl concentrations. The unzipping reaction happened for the hetero-duplexes (i.e., RNA-DNA duplexes) at low salt concentrations, on the other hand, it was not observed for the homo-duplexes at all the salt concentrations.",

author = "Ryouji Karinaga and Kazuya Koumoto and Jusaku Minari and Masami Mizu and Takahisa Anada and Seiji Shinkai and Kazuo Sakurai",

year = "2005",

language = "English",

pages = "2309",

note = "54th SPSJ Annual Meeting 2005 ; Conference date: 25-05-2005 Through 27-05-2005",

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TY - CONF

T1 - Competition between polysaccharide/polynucleotide complexation vs.polynucleotide hybridization; Salt concentration dependence of the reaction direction

AU - Karinaga, Ryouji

AU - Koumoto, Kazuya

AU - Minari, Jusaku

AU - Mizu, Masami

AU - Anada, Takahisa

AU - Shinkai, Seiji

AU - Sakurai, Kazuo

PY - 2005

Y1 - 2005

N2 - A single chain of schizophyllan (β-(1→3)-D-glucan) formed a complex with a homo-polynucleotide. In this paper, using adenine-thymine (or uracil) double strand as a model system, we explored whether s-SPG can unzip polynucleotide duplexes, examining the competition between the schizophyllan/polynucleotide complexation and the polynucleotide hybridization with circular dichroism at various NaCl concentrations. The unzipping reaction happened for the hetero-duplexes (i.e., RNA-DNA duplexes) at low salt concentrations, on the other hand, it was not observed for the homo-duplexes at all the salt concentrations.

AB - A single chain of schizophyllan (β-(1→3)-D-glucan) formed a complex with a homo-polynucleotide. In this paper, using adenine-thymine (or uracil) double strand as a model system, we explored whether s-SPG can unzip polynucleotide duplexes, examining the competition between the schizophyllan/polynucleotide complexation and the polynucleotide hybridization with circular dichroism at various NaCl concentrations. The unzipping reaction happened for the hetero-duplexes (i.e., RNA-DNA duplexes) at low salt concentrations, on the other hand, it was not observed for the homo-duplexes at all the salt concentrations.

UR - https://www.scopus.com/pages/publications/33645646676

UR - https://www.scopus.com/pages/publications/33645646676#tab=citedBy

M3 - Paper

AN - SCOPUS:33645646676

SP - 2309

T2 - 54th SPSJ Annual Meeting 2005

Y2 - 25 May 2005 through 27 May 2005

ER -

Competition between polysaccharide/polynucleotide complexation vs.polynucleotide hybridization; Salt concentration dependence of the reaction direction

Abstract

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All Science Journal Classification (ASJC) codes

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