TY - JOUR
T1 - Comparison of simultaneous quantitative analysis of methylmercury and inorganic mercury in cord blood using LC-ICP-MS and LC-CVAFS
T2 - The pilot study of the Japan environment and children’s study
AU - Iwai-Shimada, Miyuki
AU - Kobayashi, Yayoi
AU - Isobe, Tomohiko
AU - Nakayama, Shoji F.
AU - Sekiyama, Makiko
AU - Taniguchi, Yu
AU - Yamazaki, Shin
AU - Michikawa, Takehiro
AU - Oda, Masako
AU - Mitsubuchi, Hiroshi
AU - Sanefuji, Masafumi
AU - Ohga, Shouichi
AU - Mise, Nathan
AU - Ikegami, Akihiko
AU - Suga, Reiko
AU - Shimono, Masayuki
N1 - Funding Information:
The Japan Environment and Children’s Study Pilot Study was funded by the National Institute for Environmental Studies, Japan. The findings and conclusions of this article are solely the responsibility of the authors and do not represent the official views of the above institute. We are grateful to all the participants of the pilot study of the Japan Environment and Children’s Study. We would like to thank the staff of the pilot study of the Japan Environment and Children’s Study. We sincerely appreciate Hirokatsu Akagi for help with developing the method.
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/4
Y1 - 2021/4
N2 - Prenatal exposure to methylmercury (MeHg) affects child development after birth. However, many epidemiological studies have evaluated total mercury levels without analyzing speciation. Biomonitoring of MeHg and inorganic mercury (IHg) is essential to reveal each exposure level. In this study, we compared a high-throughput analysis for mercury speciation in blood using liquid chromatography-inductively coupled plasma-mass spectrometry (LC-ICP-MS) and liquid chromatography-cold vapor atomic fluorescence spectrometry (LC-CVAFS). The validated LC-ICPMS method was applied to 101 maternal blood and 366 cord blood samples in the pilot study of the Japan Environment and Children’s Study (JECS). The accuracy of the LC-CVAFS method ranged 90-115% determined by reference material analysis. To evaluate the reliability of 366 cord blood samples, fifty cord blood samples were randomly selected and analyzed using LC-CVAFS. The median (5th-95th percentile) concentrations of MeHg and IHg were 5.4 (1.9-15) and 0.33 (0.12-0.86) ng/mL, respectively, in maternal blood, and 6.3 (2.5-15) and 0.21 (0.08-0.49) ng/mL, respectively, in cord blood. Inter-laboratory comparison showed a relatively good agreement between LC-ICP-MS and LC-CVAFS. The median cord blood:maternal blood ratios of MeHg and IHg were 1.3 and 0.5, respectively. By analyzing speciation, we could focus on the health effects of each chemical form.
AB - Prenatal exposure to methylmercury (MeHg) affects child development after birth. However, many epidemiological studies have evaluated total mercury levels without analyzing speciation. Biomonitoring of MeHg and inorganic mercury (IHg) is essential to reveal each exposure level. In this study, we compared a high-throughput analysis for mercury speciation in blood using liquid chromatography-inductively coupled plasma-mass spectrometry (LC-ICP-MS) and liquid chromatography-cold vapor atomic fluorescence spectrometry (LC-CVAFS). The validated LC-ICPMS method was applied to 101 maternal blood and 366 cord blood samples in the pilot study of the Japan Environment and Children’s Study (JECS). The accuracy of the LC-CVAFS method ranged 90-115% determined by reference material analysis. To evaluate the reliability of 366 cord blood samples, fifty cord blood samples were randomly selected and analyzed using LC-CVAFS. The median (5th-95th percentile) concentrations of MeHg and IHg were 5.4 (1.9-15) and 0.33 (0.12-0.86) ng/mL, respectively, in maternal blood, and 6.3 (2.5-15) and 0.21 (0.08-0.49) ng/mL, respectively, in cord blood. Inter-laboratory comparison showed a relatively good agreement between LC-ICP-MS and LC-CVAFS. The median cord blood:maternal blood ratios of MeHg and IHg were 1.3 and 0.5, respectively. By analyzing speciation, we could focus on the health effects of each chemical form.
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U2 - 10.3390/toxics9040082
DO - 10.3390/toxics9040082
M3 - Article
AN - SCOPUS:85104858780
SN - 2305-6304
VL - 9
JO - Toxics
JF - Toxics
IS - 4
M1 - 82
ER -