Comparison of sequential derivatization with concurrent methods for GC/MS-based metabolomics

Hiromi Miyagawa, Takeshi Bamba

    Research output: Contribution to journalArticlepeer-review

    23 Citations (Scopus)


    The gas chromatography/mass spectrometry (GC/MS)-based metabolomics requires a two-step derivatization procedure consisting of oximation and silylation. However, due to the incomplete derivatization and degeneration of the metabolites, good repeatability is difficult to obtain during the batch derivatization, as the time between completing the derivatization process and GC analysis differs from sample to sample. In this research, we successfully obtained good repeatability for the peak areas of 52 selected metabolites by sequential derivatization and interval injection, in which the oximation and silylation times were maintained at constant values. In addition, the derivatization times and amount of reagents employed were varied to confirm that the optimal derivatization conditions differed for the various metabolites. In conventional batch derivatization, six metabolites, viz. glutamine, glutamic acid, histidine, alanine, asparagine, and tryptophan, exhibited fluctuations in their peak areas. Indeed, we found that for all six metabolites these differences originated from the silylation process, while the variations for glutamine and glutamic acid were related to the oximation process.

    Original languageEnglish
    Pages (from-to)160-168
    Number of pages9
    JournalJournal of Bioscience and Bioengineering
    Issue number2
    Publication statusPublished - Feb 2019

    All Science Journal Classification (ASJC) codes

    • Biotechnology
    • Bioengineering
    • Applied Microbiology and Biotechnology


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