TY - JOUR
T1 - Comparative proteomic analysis of hemolymph proteins from Autographa californica multiple nucleopolyhedrovirus (AcMNPV)-sensitive or -resistant silkworm strains during infections
AU - Xu, Jian
AU - Zhang, Pingbo
AU - Kusakabe, Takahiro
AU - Mon, Hiroaki
AU - Li, Zhiqing
AU - Zhu, Li
AU - Iiyama, Kazuhiro
AU - Banno, Yutaka
AU - Morokuma, Daisuke
AU - Lee, Jae Man
N1 - Funding Information:
We thank Dr. Aoki (Kyushu University Graduate School) for providing the Bm5 cell line. This work was supported in part by the Program for Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN) . The cost of publication was supported in part by the Research Grant for Young Investigators of Faculty of Agriculture, Kyushu University . JX was supported by a JSPS Postdoctoral Fellowship (26-14090). The authors have declared that no conflict of interest exists.
PY - 2015/12/1
Y1 - 2015/12/1
N2 - Abstract We reported previously that baculovirus AcMNPV host-ranges in silkworm strains are controlled by a novel third chromosomal locus. To further isolate the potential host factor and uncover the functional pathway involved, in this study we analyzed hemolymph proteins from AcMNPV-resistant or -sensitive silkworm strains infected with baculoviruses. All the protein spots from 2D electrophoresis were characterized by MALDI-TOF MS and further systematically assessed for differentially regulated proteins at different stages of infection. Subsequently, six candidates were selected for functional analysis using Bm5 cells, where the candidates were knocked-down or overexpressed. We observed that mRNA expression levels of beta-N-acetylglucosaminidase and prophenoloxidase subunit 2 are significantly upregulated during AcMNPV infections in Bm5 cells. Ultimately, we found that RNA interference of ribosomal protein RpL34 causes serious damages to cell viability as well as abortive infection, indicating that ribosomal components are essential for productive baculovirus infection.
AB - Abstract We reported previously that baculovirus AcMNPV host-ranges in silkworm strains are controlled by a novel third chromosomal locus. To further isolate the potential host factor and uncover the functional pathway involved, in this study we analyzed hemolymph proteins from AcMNPV-resistant or -sensitive silkworm strains infected with baculoviruses. All the protein spots from 2D electrophoresis were characterized by MALDI-TOF MS and further systematically assessed for differentially regulated proteins at different stages of infection. Subsequently, six candidates were selected for functional analysis using Bm5 cells, where the candidates were knocked-down or overexpressed. We observed that mRNA expression levels of beta-N-acetylglucosaminidase and prophenoloxidase subunit 2 are significantly upregulated during AcMNPV infections in Bm5 cells. Ultimately, we found that RNA interference of ribosomal protein RpL34 causes serious damages to cell viability as well as abortive infection, indicating that ribosomal components are essential for productive baculovirus infection.
UR - http://www.scopus.com/inward/record.url?scp=84939213412&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84939213412&partnerID=8YFLogxK
U2 - 10.1016/j.cbd.2015.07.003
DO - 10.1016/j.cbd.2015.07.003
M3 - Article
C2 - 26276225
AN - SCOPUS:84939213412
SN - 1744-117X
VL - 16
SP - 36
EP - 47
JO - Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics
JF - Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics
M1 - 371
ER -