TY - JOUR
T1 - Clinical significance of the reduced expression of G protein gamma 7 (GNG7) in oesophageal cancer
AU - Ohta, M.
AU - Mimori, K.
AU - Fukuyoshi, Y.
AU - Kita, Y.
AU - Motoyama, K.
AU - Yamashita, K.
AU - Ishii, H.
AU - Inoue, H.
AU - Mori, M.
N1 - Funding Information:
We thank Dr Yasuyuki and Ms Akiko Ochi (Division of Disease Genes, Research Center for Genetic Information, Medical Institute of Bioregulation, Kyushu University) for providing us the technology of pyrosequencing analysis and for helpful discussion. We are grateful to Ms Toshiko Simo-oka, Ms Kazue Ogata, Ms Haruko Yasunami, Ms Mayumi Oda and Ms Michiko Kasagi for their excellent technical assistance. We appreciate Dr Shimada, Department of Surgery and Basic Surgical Research, Kyoto University, for providing the cell lines. This work was supported in part by the following grants and foundations: a Grant-in-Aid for Scientific Research (S) (17109013), Kanae Foundation for Life and Socio-Medical Science in 2006, CREST, Japan Science and Technology Agency (JST) and Japan Society for the Promotion of Science (JSPS; Grant no. 19390336).
PY - 2008/1/29
Y1 - 2008/1/29
N2 - We previously cloned human G protein gamma 7 (GNG7) and demonstrated that it was downregulated in gastrointestinal cancer. The significance of GNG7 expression in oesophageal cancer is unknown. TaqMan quantitative real-time PCR was performed to determine the clinical significance of GNG7 expression in 55 cases of oesophageal cancer. Furthermore, GNG7-transfected oesophageal cancer cells were analysed in laboratory studies at genomic and epigenetic levels. Twenty-seven patients with low GNG7 expression showed significantly poorer survival than did 28 patients with high expression (P<0.05). Tumours with low GNG7 expression invaded deeper than those with high GNG7 expression (P<0.05), both in vivo and in vitro. Eight tumours retained GNG7 expression, and they did not show either promoter hypermethylation or loss of heterozygosity (LOH). In 38 tumours with GNG7 suppression, 22 (57%) showed either LOH or promoter hypermethylation. In addition, GNG7 expression was significantly associated with the presence of miR328 in oesophageal cancer cell lines, which suggests that this microRNA might be a regulator of GNG7 expression. GNG7 suppression represents a new prognostic indicator in cases of oesophageal cancer. GNG7 might be suppressed by LOH and promoter hypermethylation or by microRNA.
AB - We previously cloned human G protein gamma 7 (GNG7) and demonstrated that it was downregulated in gastrointestinal cancer. The significance of GNG7 expression in oesophageal cancer is unknown. TaqMan quantitative real-time PCR was performed to determine the clinical significance of GNG7 expression in 55 cases of oesophageal cancer. Furthermore, GNG7-transfected oesophageal cancer cells were analysed in laboratory studies at genomic and epigenetic levels. Twenty-seven patients with low GNG7 expression showed significantly poorer survival than did 28 patients with high expression (P<0.05). Tumours with low GNG7 expression invaded deeper than those with high GNG7 expression (P<0.05), both in vivo and in vitro. Eight tumours retained GNG7 expression, and they did not show either promoter hypermethylation or loss of heterozygosity (LOH). In 38 tumours with GNG7 suppression, 22 (57%) showed either LOH or promoter hypermethylation. In addition, GNG7 expression was significantly associated with the presence of miR328 in oesophageal cancer cell lines, which suggests that this microRNA might be a regulator of GNG7 expression. GNG7 suppression represents a new prognostic indicator in cases of oesophageal cancer. GNG7 might be suppressed by LOH and promoter hypermethylation or by microRNA.
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U2 - 10.1038/sj.bjc.6604124
DO - 10.1038/sj.bjc.6604124
M3 - Article
C2 - 18219292
AN - SCOPUS:38549163855
SN - 0007-0920
VL - 98
SP - 410
EP - 417
JO - British journal of cancer
JF - British journal of cancer
IS - 2
ER -
