Clinical significance of K-ras mutations in intraoperative tumor drainage blood from patients with colorectal carcinoma

Tsuyoshi Etoh, Hiroaki Ueo, Hiroshi Inoue, Hiroshi Sato, Tohru Utsunomiya, Graham F. Barnard, Seigo Kitano, Masaki Mori

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


Background: Recurrent and metastatic carcinoma of the colorectum remains a major problem. This may be ascribed to the presence of micrometastasis at diagnosis. The purpose of this study was to analyze prospectively the clinical value of detecting K-ras mutations in the perioperative circulating blood from patients with colorectal carcinoma. Methods: Twenty-four patients whose tumor carried mutations in codon 12 of the K-ras gene were studied for the presence of cancer cells in perioperative blood samples, in particular, tumor drainage samples. A detection assay using CD45 immunomagnetic separation plus nested mutant allele specific amplification (MASA) was performed. Results: K-ras mutations in CD45 negative cells in tumor drainage blood were detected in 7 (29.2%) of 24 patients. There was no significant relationship between the presence of a K-ras mutation and clinicopathological features. Four (57.1%) of the seven patients with a positive K-ras mutation in drainage blood had early recurrent disease. Of the 17 patients with no K-ras mutation, none developed metastatic disease. The recurrence rate of the K-ras mutation positive group was higher than that of the K-ras mutation negative group (P < 01). There was a significant difference, regarding prognosis, between K-ras mutation positive and negative groups (P < .01). Conclusions: This preliminary study demonstrates that the detection of circulating cancer cells in the tumor drainage blood by our new assay system may provide a predictor of recurrence and metastasis of colorectal cancer.

Original languageEnglish
Pages (from-to)407-412
Number of pages6
JournalAnnals of Surgical Oncology
Issue number5
Publication statusPublished - 2001
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Surgery
  • Oncology


Dive into the research topics of 'Clinical significance of K-ras mutations in intraoperative tumor drainage blood from patients with colorectal carcinoma'. Together they form a unique fingerprint.

Cite this