TY - JOUR
T1 - Clinical significance of cytokeratin positive cells in bone marrow of gastric cancer patients
AU - Oki, Eiji
AU - Kakeji, Yoshihiro
AU - Baba, Hideo
AU - Nishida, Kojiro
AU - Koga, Tadashi
AU - Tokunaga, Eriko
AU - Egashira, Akinori
AU - Ikeda, Keisuke
AU - Yoshida, Rintaro
AU - Yamamoto, Manabu
AU - Morita, Masaru
AU - Maehara, Yoshihiko
N1 - Funding Information:
Acknowledgments We are grateful to J. Tsuchihashi and K. Miyam-oto for technical assistance. This study was supported by grants from the Ministry of Education, Science, Sports and Culture of Japan.
PY - 2007/12
Y1 - 2007/12
N2 - Bone marrow (BM) is a prognostically relevant indicator organ of micrometastasis; however, the clinical importance of BM micrometastasis in gastric cancer patients is not yet known. In the present study, the BM of 267 consecutive patients with primary gastric cancer was examined for tumor cells using immunocytochemical techniques. Real-time quantitative RT-PCR was used to ensure that the tumor cells were detected properly. Among the 267 cases analyzed, 30 cases (11.2%) had cytokeratine-positive cells in the bone marrow. Positive findings were related to the tumor stage (P < 0.05) and to the prominent depth of invasion (P < 0.05). All patients with liver metastasis at operation had cytokeratine-positive cells in the BM. Recurrence of the disease was confirmed in 50 cases (18.7%); 4 of 30 (13.3%) in the cytokeratine-positive group and 46 of 237 (19.4%) in the cytokeratine-negative group. There were no significant differences in the 5-year survival rates between the cytokeratine-positive and cytokeratin-negative groups. Our study shows that BM micrometastasis increases according to tumor progression; however, only a subset of cancer cells may survive in the BM and finally evolve to a clinically apparent disease. Therefore it does not accurately predict the prognosis or recurrence of the disease.
AB - Bone marrow (BM) is a prognostically relevant indicator organ of micrometastasis; however, the clinical importance of BM micrometastasis in gastric cancer patients is not yet known. In the present study, the BM of 267 consecutive patients with primary gastric cancer was examined for tumor cells using immunocytochemical techniques. Real-time quantitative RT-PCR was used to ensure that the tumor cells were detected properly. Among the 267 cases analyzed, 30 cases (11.2%) had cytokeratine-positive cells in the bone marrow. Positive findings were related to the tumor stage (P < 0.05) and to the prominent depth of invasion (P < 0.05). All patients with liver metastasis at operation had cytokeratine-positive cells in the BM. Recurrence of the disease was confirmed in 50 cases (18.7%); 4 of 30 (13.3%) in the cytokeratine-positive group and 46 of 237 (19.4%) in the cytokeratine-negative group. There were no significant differences in the 5-year survival rates between the cytokeratine-positive and cytokeratin-negative groups. Our study shows that BM micrometastasis increases according to tumor progression; however, only a subset of cancer cells may survive in the BM and finally evolve to a clinically apparent disease. Therefore it does not accurately predict the prognosis or recurrence of the disease.
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U2 - 10.1007/s00432-007-0258-1
DO - 10.1007/s00432-007-0258-1
M3 - Article
C2 - 17588173
AN - SCOPUS:35248815430
SN - 0171-5216
VL - 133
SP - 995
EP - 1000
JO - Journal of Cancer Research and Clinical Oncology
JF - Journal of Cancer Research and Clinical Oncology
IS - 12
ER -
