TY - JOUR
T1 - Clinical and biological impact of cyclin-dependent kinase subunit 2 in esophageal squamous cell carcinoma
AU - Kita, Yoshiaki
AU - Nishizono, Yuka
AU - Okumura, Hiroshi
AU - Uchikado, Yasuto
AU - Sasaki, Ken
AU - Matsumoto, Masataka
AU - Setoyama, Tetsuro
AU - Tanoue, Kiyonori
AU - Omoto, Itaru
AU - Mori, Shinichiro
AU - Owaki, Tetsuhiro
AU - Ishigami, Sumiya
AU - Nakagawa, Hiroshi
AU - Tanaka, Fumiaki
AU - Mimori, Koshi
AU - Mori, Masaki
AU - Natsugoe, Shoji
PY - 2014/5
Y1 - 2014/5
N2 - Cyclin-dependent kinase subunit 2 (CKS2) is a cyclin-dependent kinase subunit (CKS) family member that participates in cell cycle regulation. Few studies have investigated its involvement in esophageal squamous cell carcinoma (ESCC). The aim of the present study was to assess the clinical significance of CKS2 in ESCC. We used immunohistochemistry to study the clinicopathologic significance of CKS2 protein expression in 121 patients with ESCC. Using real-time reverse transcriptase-polymerase chain reaction (RT-PCR), we examined the expression of CKS2 mRNA in tumors and the corresponding normal esophageal tissues that were obtained from 62 patients. Finally, siRNA-mediated attenuation of CKS2 expression was examined in vitro. CKS2 protein expression was significantly correlated with depth of tumor invasion, clinical stage, lymphatic invasion and distant metastasis (p=0.033, 0.028, 0.041 and 0.009, respectively). CKS2 mRNA expression was higher in cancer tissue than in corresponding normal tissue (p<0.001). Patients with positive-CKS2 protein.
AB - Cyclin-dependent kinase subunit 2 (CKS2) is a cyclin-dependent kinase subunit (CKS) family member that participates in cell cycle regulation. Few studies have investigated its involvement in esophageal squamous cell carcinoma (ESCC). The aim of the present study was to assess the clinical significance of CKS2 in ESCC. We used immunohistochemistry to study the clinicopathologic significance of CKS2 protein expression in 121 patients with ESCC. Using real-time reverse transcriptase-polymerase chain reaction (RT-PCR), we examined the expression of CKS2 mRNA in tumors and the corresponding normal esophageal tissues that were obtained from 62 patients. Finally, siRNA-mediated attenuation of CKS2 expression was examined in vitro. CKS2 protein expression was significantly correlated with depth of tumor invasion, clinical stage, lymphatic invasion and distant metastasis (p=0.033, 0.028, 0.041 and 0.009, respectively). CKS2 mRNA expression was higher in cancer tissue than in corresponding normal tissue (p<0.001). Patients with positive-CKS2 protein.
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U2 - 10.3892/or.2014.3062
DO - 10.3892/or.2014.3062
M3 - Article
C2 - 24604089
AN - SCOPUS:84898605721
SN - 1021-335X
VL - 31
SP - 1986
EP - 1992
JO - Oncology reports
JF - Oncology reports
IS - 5
ER -
