Cisplatin blocks mechano-electric transducer current in chick cochlear hair cells

Takashi Kimitsuki; Takashi Nakagawa; Kazutaka Hisashi; Shizuo Komune; Sohtaro Komiyama

doi:10.1016/0378-5955(93)90021-R

Cisplatin blocks mechano-electric transducer current in chick cochlear hair cells

Takashi Kimitsuki
, Takashi Nakagawa
, Kazutaka Hisashi
, Shizuo Komune
, Sohtaro Komiyama

Surgery

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

The effects of cisplatin (cis-dichlorodiammine platinum II, CDDP) on the mechano-electrical transduction (MET) current were investigated with a whole-cell patch-electrode voltage clamp technique in dissociated cochlear hair cells of chicks. CDDP blocked the MET channel in a dose-and voltage-dependent manner. At -50 mV, CDDP blocked the MET channel with a Hill coefficient of approximately 2 and a dissociation constant (K_D) of 1.5×10^-3 M. The kinetics of CDDP blockade consist of a voltage-independent and a voltage-dependent component.

Original language	English
Pages (from-to)	64-68
Number of pages	5
Journal	Hearing Research
Volume	71
Issue number	1-2
DOIs	https://doi.org/10.1016/0378-5955(93)90021-R
Publication status	Published - Dec 1993

All Science Journal Classification (ASJC) codes

Sensory Systems

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10.1016/0378-5955(93)90021-R

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title = "Cisplatin blocks mechano-electric transducer current in chick cochlear hair cells",

abstract = "The effects of cisplatin (cis-dichlorodiammine platinum II, CDDP) on the mechano-electrical transduction (MET) current were investigated with a whole-cell patch-electrode voltage clamp technique in dissociated cochlear hair cells of chicks. CDDP blocked the MET channel in a dose-and voltage-dependent manner. At -50 mV, CDDP blocked the MET channel with a Hill coefficient of approximately 2 and a dissociation constant (KD) of 1.5×10-3 M. The kinetics of CDDP blockade consist of a voltage-independent and a voltage-dependent component.",

keywords = "Cisplatin, Hair cell, Mechano-electric transduction channel, Ototoxicity, Patch clamp technique",

author = "Takashi Kimitsuki and Takashi Nakagawa and Kazutaka Hisashi and Shizuo Komune and Sohtaro Komiyama",

year = "1993",

month = dec,

doi = "10.1016/0378-5955(93)90021-R",

language = "English",

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journal = "Hearing Research",

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TY - JOUR

T1 - Cisplatin blocks mechano-electric transducer current in chick cochlear hair cells

AU - Kimitsuki, Takashi

AU - Nakagawa, Takashi

AU - Hisashi, Kazutaka

AU - Komune, Shizuo

AU - Komiyama, Sohtaro

PY - 1993/12

Y1 - 1993/12

N2 - The effects of cisplatin (cis-dichlorodiammine platinum II, CDDP) on the mechano-electrical transduction (MET) current were investigated with a whole-cell patch-electrode voltage clamp technique in dissociated cochlear hair cells of chicks. CDDP blocked the MET channel in a dose-and voltage-dependent manner. At -50 mV, CDDP blocked the MET channel with a Hill coefficient of approximately 2 and a dissociation constant (KD) of 1.5×10-3 M. The kinetics of CDDP blockade consist of a voltage-independent and a voltage-dependent component.

AB - The effects of cisplatin (cis-dichlorodiammine platinum II, CDDP) on the mechano-electrical transduction (MET) current were investigated with a whole-cell patch-electrode voltage clamp technique in dissociated cochlear hair cells of chicks. CDDP blocked the MET channel in a dose-and voltage-dependent manner. At -50 mV, CDDP blocked the MET channel with a Hill coefficient of approximately 2 and a dissociation constant (KD) of 1.5×10-3 M. The kinetics of CDDP blockade consist of a voltage-independent and a voltage-dependent component.

KW - Cisplatin

KW - Hair cell

KW - Mechano-electric transduction channel

KW - Ototoxicity

KW - Patch clamp technique

UR - https://www.scopus.com/pages/publications/0027762811

UR - https://www.scopus.com/pages/publications/0027762811#tab=citedBy

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DO - 10.1016/0378-5955(93)90021-R

M3 - Article

C2 - 8113146

AN - SCOPUS:0027762811

SN - 0378-5955

VL - 71

SP - 64

EP - 68

JO - Hearing Research

JF - Hearing Research

IS - 1-2

ER -

Cisplatin blocks mechano-electric transducer current in chick cochlear hair cells

Abstract

All Science Journal Classification (ASJC) codes

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