Circular dichroism and absorption spectra of haemoglobin Bart's

Takehiko Sasazuki; Akio Isomoto; Hiroshi Nakajima

doi:10.1016/0022-2836(72)90287-2

Circular dichroism and absorption spectra of haemoglobin Bart's

Takehiko Sasazuki, Akio Isomoto, Hiroshi Nakajima

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Circular dichroism spectra of haemoglobin Bart's (γ₄) in the region from 240 to 600 nm were different from those of human adult haemoglobin, but closely similar to those of the β-subunit of human adult haemoglobin. The amplitude of the positive circular dichroism maximum of deoxygenated haemoglobin Bart's in the Soret region was much less than that of human adult haemoglobin. The peak molar extinction coefficient of deoxygenated haemoglobin Bart's in the Soret region was found to be lower than that of deoxygenated human adult haemoglobin. These data indicate that haemoglobin Bart's, which is composed of four identical chains and lacks co-operativity, is structurally similar to haemoglobin H (β₄).

Original language	English
Pages (from-to)	365-369
Number of pages	5
Journal	Journal of Molecular Biology
Volume	65
Issue number	2
DOIs	https://doi.org/10.1016/0022-2836(72)90287-2
Publication status	Published - Mar 28 1972
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Molecular Biology

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10.1016/0022-2836(72)90287-2

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title = "Circular dichroism and absorption spectra of haemoglobin Bart's",

abstract = "Circular dichroism spectra of haemoglobin Bart's (γ4) in the region from 240 to 600 nm were different from those of human adult haemoglobin, but closely similar to those of the β-subunit of human adult haemoglobin. The amplitude of the positive circular dichroism maximum of deoxygenated haemoglobin Bart's in the Soret region was much less than that of human adult haemoglobin. The peak molar extinction coefficient of deoxygenated haemoglobin Bart's in the Soret region was found to be lower than that of deoxygenated human adult haemoglobin. These data indicate that haemoglobin Bart's, which is composed of four identical chains and lacks co-operativity, is structurally similar to haemoglobin H (β4).",

author = "Takehiko Sasazuki and Akio Isomoto and Hiroshi Nakajima",

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doi = "10.1016/0022-2836(72)90287-2",

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T1 - Circular dichroism and absorption spectra of haemoglobin Bart's

AU - Sasazuki, Takehiko

AU - Isomoto, Akio

AU - Nakajima, Hiroshi

PY - 1972/3/28

Y1 - 1972/3/28

N2 - Circular dichroism spectra of haemoglobin Bart's (γ4) in the region from 240 to 600 nm were different from those of human adult haemoglobin, but closely similar to those of the β-subunit of human adult haemoglobin. The amplitude of the positive circular dichroism maximum of deoxygenated haemoglobin Bart's in the Soret region was much less than that of human adult haemoglobin. The peak molar extinction coefficient of deoxygenated haemoglobin Bart's in the Soret region was found to be lower than that of deoxygenated human adult haemoglobin. These data indicate that haemoglobin Bart's, which is composed of four identical chains and lacks co-operativity, is structurally similar to haemoglobin H (β4).

AB - Circular dichroism spectra of haemoglobin Bart's (γ4) in the region from 240 to 600 nm were different from those of human adult haemoglobin, but closely similar to those of the β-subunit of human adult haemoglobin. The amplitude of the positive circular dichroism maximum of deoxygenated haemoglobin Bart's in the Soret region was much less than that of human adult haemoglobin. The peak molar extinction coefficient of deoxygenated haemoglobin Bart's in the Soret region was found to be lower than that of deoxygenated human adult haemoglobin. These data indicate that haemoglobin Bart's, which is composed of four identical chains and lacks co-operativity, is structurally similar to haemoglobin H (β4).

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Circular dichroism and absorption spectra of haemoglobin Bart's

Abstract

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