TY - JOUR
T1 - Circadian changes of d-alanine and related compounds in rats and the effect of restricted feeding on their amounts
AU - Morikawa, Akiko
AU - Hamase, Kenji
AU - Miyoshi, Yurika
AU - Koyanagi, Satoru
AU - Ohdo, Shigehiro
AU - Zaitsu, Kiyoshi
N1 - Funding Information:
This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan, Takeda Science Foundation, and Industrial Technology Research Grant Program in ’05 from New Energy and Industrial Technology Development Organization (NEDO) of Japan. The authors appreciate Shiseido Co. Ltd. (Tokyo, Japan) for providing the HPLC system (NANOSPACE series) and the technical support.
PY - 2008/11/1
Y1 - 2008/11/1
N2 - The circadian changes of d-alanine (d-Ala), an intrinsic d-amino acid found in mammals, were investigated in rats with diurnal and nocturnal habits, and the profiles were compared to those of l-Ala, other d-amino acids and several hormones. Determination of d-Ala in the rat plasma, pancreas and anterior pituitary gland was carried out using a sensitive and selective two-dimensional HPLC system combining a micro-ODS column and an enantioselective column after fluorescence derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F). The amount of d-Ala was high during the sleeping period and low during the active period in rats with both diurnal and nocturnal habits, indicating for the first time that the d-Ala is closely related to the activity rhythm of animals. In contrast, l-Ala and other d-amino acids did not show any clear circadian changes. The circadian change of d-Ala inversely correlated with that of the plasma insulin level in rats with both diurnal and nocturnal habits. Considered together with our previous findings that d-Ala is localized in the insulin secreting beta-cells in the rat pancreas, it is strongly suggested that d-Ala has some functional relationships to insulin in mammals.
AB - The circadian changes of d-alanine (d-Ala), an intrinsic d-amino acid found in mammals, were investigated in rats with diurnal and nocturnal habits, and the profiles were compared to those of l-Ala, other d-amino acids and several hormones. Determination of d-Ala in the rat plasma, pancreas and anterior pituitary gland was carried out using a sensitive and selective two-dimensional HPLC system combining a micro-ODS column and an enantioselective column after fluorescence derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F). The amount of d-Ala was high during the sleeping period and low during the active period in rats with both diurnal and nocturnal habits, indicating for the first time that the d-Ala is closely related to the activity rhythm of animals. In contrast, l-Ala and other d-amino acids did not show any clear circadian changes. The circadian change of d-Ala inversely correlated with that of the plasma insulin level in rats with both diurnal and nocturnal habits. Considered together with our previous findings that d-Ala is localized in the insulin secreting beta-cells in the rat pancreas, it is strongly suggested that d-Ala has some functional relationships to insulin in mammals.
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U2 - 10.1016/j.jchromb.2008.04.004
DO - 10.1016/j.jchromb.2008.04.004
M3 - Article
C2 - 18448397
AN - SCOPUS:54049155112
SN - 1570-0232
VL - 875
SP - 168
EP - 173
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
IS - 1
ER -