Chromosomal assignment of the chicken NP220 gene encoding a large DNA-binding nuclear protein and its targeted disruption in chicken DT40 cells

Yuichi Matsushima; Kiyoshi Matsumura; Shohji Ishii; Tomohiro Suzuki; Yoichi Matsuda; Yasuo Kitagawa

doi:10.1023/a:1008107227862

Chromosomal assignment of the chicken NP220 gene encoding a large DNA-binding nuclear protein and its targeted disruption in chicken DT40 cells

Yuichi Matsushima, Kiyoshi Matsumura, Shohji Ishii, Tomohiro Suzuki, Yoichi Matsuda, Yasuo Kitagawa

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

NP220s form a family of DNA-binding nuclear proteins originally found in human cell lines. Four isoforms of NP220 are produced in humans and mice probably by alternative splicing of two sequence units. They have RNA-recognition and arginine/serine rich motives commonly found in many nuclear proteins important for pre-mRNA processing. In order to analyze the function of NP220s, its gene was disrupted in chicken cell line DT40. For this, gemomic DNA clone of chicken NP220 was isolated and the gene was localized to chromosome 4q2.7-q2.8. Chicken NP220 conserves MH1 and zinc finger-like motives found in human and mouse NP220s. Despite its expression as a mRNA of 6 kb in wild type DT40 cells, disruption of the NP220 gene did not impair the growth of DT40 cells.

Original language	English
Pages (from-to)	109-115
Number of pages	7
Journal	Cytotechnology
Volume	33
Issue number	1-3
DOIs	https://doi.org/10.1023/a:1008107227862
Publication status	Published - 2000
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Biomedical Engineering
Clinical Biochemistry
Cell Biology

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title = "Chromosomal assignment of the chicken NP220 gene encoding a large DNA-binding nuclear protein and its targeted disruption in chicken DT40 cells",

abstract = "NP220s form a family of DNA-binding nuclear proteins originally found in human cell lines. Four isoforms of NP220 are produced in humans and mice probably by alternative splicing of two sequence units. They have RNA-recognition and arginine/serine rich motives commonly found in many nuclear proteins important for pre-mRNA processing. In order to analyze the function of NP220s, its gene was disrupted in chicken cell line DT40. For this, gemomic DNA clone of chicken NP220 was isolated and the gene was localized to chromosome 4q2.7-q2.8. Chicken NP220 conserves MH1 and zinc finger-like motives found in human and mouse NP220s. Despite its expression as a mRNA of 6 kb in wild type DT40 cells, disruption of the NP220 gene did not impair the growth of DT40 cells.",

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T1 - Chromosomal assignment of the chicken NP220 gene encoding a large DNA-binding nuclear protein and its targeted disruption in chicken DT40 cells

AU - Matsushima, Yuichi

AU - Matsumura, Kiyoshi

AU - Ishii, Shohji

AU - Suzuki, Tomohiro

AU - Matsuda, Yoichi

AU - Kitagawa, Yasuo

PY - 2000

Y1 - 2000

N2 - NP220s form a family of DNA-binding nuclear proteins originally found in human cell lines. Four isoforms of NP220 are produced in humans and mice probably by alternative splicing of two sequence units. They have RNA-recognition and arginine/serine rich motives commonly found in many nuclear proteins important for pre-mRNA processing. In order to analyze the function of NP220s, its gene was disrupted in chicken cell line DT40. For this, gemomic DNA clone of chicken NP220 was isolated and the gene was localized to chromosome 4q2.7-q2.8. Chicken NP220 conserves MH1 and zinc finger-like motives found in human and mouse NP220s. Despite its expression as a mRNA of 6 kb in wild type DT40 cells, disruption of the NP220 gene did not impair the growth of DT40 cells.

AB - NP220s form a family of DNA-binding nuclear proteins originally found in human cell lines. Four isoforms of NP220 are produced in humans and mice probably by alternative splicing of two sequence units. They have RNA-recognition and arginine/serine rich motives commonly found in many nuclear proteins important for pre-mRNA processing. In order to analyze the function of NP220s, its gene was disrupted in chicken cell line DT40. For this, gemomic DNA clone of chicken NP220 was isolated and the gene was localized to chromosome 4q2.7-q2.8. Chicken NP220 conserves MH1 and zinc finger-like motives found in human and mouse NP220s. Despite its expression as a mRNA of 6 kb in wild type DT40 cells, disruption of the NP220 gene did not impair the growth of DT40 cells.

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Chromosomal assignment of the chicken NP220 gene encoding a large DNA-binding nuclear protein and its targeted disruption in chicken DT40 cells

Abstract

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