TY - JOUR
T1 - Chicken immunoglobulin μ-chain gene
T2 - Germline organization and tandem repeats characteristic of class switch recombination
AU - Kitao, Hiroyuki
AU - Arakawa, Hiroshi
AU - Yamagishi, Hideo
AU - Shimizu, Akira
N1 - Funding Information:
We are gratefult o Drs. Y. Hirota and S. Furusawa for providing the H-B 15 line of chicken,a nd to Dr. A. Reynaudf or the J, regionc lone.W e also thank Mr. K. Tsubokura for preparationo f cDNA probes, and Dr. M. Nazareaf or critical readingo f this manuscriptT. his work was supportedi n part by Grants-in-Aid for Sci-enceR esearchf rom the Ministry of Education,S cience, Culture and Sports of Japan.
PY - 1996/9
Y1 - 1996/9
N2 - We have isolated the phage clones covering the region spanning from the heavy (H)-chain joining (J) region to the end of the μ-chain gene of the chicken immunoglobulin (Ig). The distance from J(H) to the first exon of the μ-chain constant (C) region is approximately 13 kb, and introns between the C region exons measure more than 3 kb. These distances are significantly larger than those of known μ-chain genes. We found a region cross-hybridizing to the switch regions of the mouse Cμ and Cα genes just in front of the first exon of Cμ. Partial nucleotide sequencing of this region revealed that this region consists of tandem repeats of pentamers (C/T)(C/A)CAG complementary to the mammalian switch repetitive region. These findings suggest that this region is a good candidate for a class switch region of the μ-chain gene of chicken Ig.
AB - We have isolated the phage clones covering the region spanning from the heavy (H)-chain joining (J) region to the end of the μ-chain gene of the chicken immunoglobulin (Ig). The distance from J(H) to the first exon of the μ-chain constant (C) region is approximately 13 kb, and introns between the C region exons measure more than 3 kb. These distances are significantly larger than those of known μ-chain genes. We found a region cross-hybridizing to the switch regions of the mouse Cμ and Cα genes just in front of the first exon of Cμ. Partial nucleotide sequencing of this region revealed that this region consists of tandem repeats of pentamers (C/T)(C/A)CAG complementary to the mammalian switch repetitive region. These findings suggest that this region is a good candidate for a class switch region of the μ-chain gene of chicken Ig.
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U2 - 10.1016/0165-2478(96)02592-8
DO - 10.1016/0165-2478(96)02592-8
M3 - Article
C2 - 8905403
AN - SCOPUS:0030249125
SN - 0165-2478
VL - 52
SP - 99
EP - 104
JO - Immunology Letters
JF - Immunology Letters
IS - 2-3
ER -