TY - JOUR
T1 - Characterization of long-term endogenous cardiac repair in children after heart transplantation
AU - Rupp, Stefan
AU - Koyanagi, Masamichi
AU - Iwasaki, Masayoshi
AU - Bauer, Jürgen
AU - Von Gerlach, Susanne
AU - Schranz, Dietmar
AU - Zeiher, Andreas M.
AU - Dimmeler, Stefanie
N1 - Funding Information:
This work was supported by the Excellence Cluster Cardiopulmonary Systems (EXC 14711) by the Deutsche Forschungsgemeinschaft.
PY - 2008/8
Y1 - 2008/8
N2 - Aims: Circulating cells repopulate the heart at a very low rate in adult humans. The knowledge about time-dependent cardiac regeneration is very limited and the contribution of circulating cells to cardiomyocytes or vascular cells in children is unknown. This study investigates the endogenous repair capacity and the long-term incorporation of circulating cells in heart-transplanted children. Methods and results: Cardiac and endothelial chimerism was detected in endomyocardial biopsies of nine children (age 1 months-14 years) with sex-mismatched heart transplantation by fluorescence in situ hybridization. Time from transplantation to biopsy ranged from 1 month up to 10 years. The extent of repopulating cardiomyocytes was 2.39 ± 1.54% (range: 0-4.2%) and correlated significantly with the time from transplantation to biopsy sampling (r2 = 0.69, P = 0.006; n = 9). The calculated contribution of male cardiomyocytes in the female heart per year was 0.36 ± 0.09%. Consistent with the previous reports, the incorporation of vascular cells was higher compared with cardiomyocytes (14.4 ± 4.17%), but did not correlate in a time-dependent manner. Conclusion: Circulating cells contribute to cardiomyocytes and endothelial cells in children after heart transplantation. The incidence of repopulating cardiomyocytes continuously increases in a time-dependent manner (∼4% Y-chromosome+ cardiomyocytes/10 years) and resembles the cardiac regeneration activity observed in adults.
AB - Aims: Circulating cells repopulate the heart at a very low rate in adult humans. The knowledge about time-dependent cardiac regeneration is very limited and the contribution of circulating cells to cardiomyocytes or vascular cells in children is unknown. This study investigates the endogenous repair capacity and the long-term incorporation of circulating cells in heart-transplanted children. Methods and results: Cardiac and endothelial chimerism was detected in endomyocardial biopsies of nine children (age 1 months-14 years) with sex-mismatched heart transplantation by fluorescence in situ hybridization. Time from transplantation to biopsy ranged from 1 month up to 10 years. The extent of repopulating cardiomyocytes was 2.39 ± 1.54% (range: 0-4.2%) and correlated significantly with the time from transplantation to biopsy sampling (r2 = 0.69, P = 0.006; n = 9). The calculated contribution of male cardiomyocytes in the female heart per year was 0.36 ± 0.09%. Consistent with the previous reports, the incorporation of vascular cells was higher compared with cardiomyocytes (14.4 ± 4.17%), but did not correlate in a time-dependent manner. Conclusion: Circulating cells contribute to cardiomyocytes and endothelial cells in children after heart transplantation. The incidence of repopulating cardiomyocytes continuously increases in a time-dependent manner (∼4% Y-chromosome+ cardiomyocytes/10 years) and resembles the cardiac regeneration activity observed in adults.
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U2 - 10.1093/eurheartj/ehn223
DO - 10.1093/eurheartj/ehn223
M3 - Article
C2 - 18511408
AN - SCOPUS:48749117667
SN - 0195-668X
VL - 29
SP - 1867
EP - 1872
JO - European heart journal
JF - European heart journal
IS - 15
ER -