TY - JOUR
T1 - Characterization of a novel thogotovirus isolated from Amblyomma testudinarium ticks in Ehime, Japan
T2 - A significant phylogenetic relationship to Bourbon virus
AU - Ejiri, Hiroko
AU - Lim, Chang Kweng
AU - Isawa, Haruhiko
AU - Fujita, Ryosuke
AU - Murota, Katsunori
AU - Sato, Tomomi
AU - Kobayashi, Daisuke
AU - Kan, Miki
AU - Hattori, Masashi
AU - Kimura, Toshiya
AU - Yamaguchi, Yukie
AU - Takayama-Ito, Mutsuyo
AU - Horiya, Madoka
AU - Posadas-Herrera, Guillermo
AU - Minami, Shohei
AU - Kuwata, Ryusei
AU - Shimoda, Hiroshi
AU - Maeda, Ken
AU - Katayama, Yukie
AU - Mizutani, Tetsuya
AU - Saijo, Masayuki
AU - Kaku, Koki
AU - Shinomiya, Hiroto
AU - Sawabe, Kyoko
N1 - Funding Information:
This study was supported by grants-in-aid of Japanese Ministry of Health, Labor and Welfare (H24-Shinko-Ippan-007 and Regulatory Science Research), JSPS KAKENHI (Grant Numbers 21406012, 25305010, 25893151 and 12J09845), and the Research Program on Emerging and Re-emerging Infectious Diseases from Japan Agency for Medical Research and development, AMED. The authors would like to thank Enago (www.enago.jp) for the English language review. Conflicts of interest: none.
Funding Information:
This study was supported by grants-in-aid of Japanese Ministry of Health, Labor and Welfare (H24-Shinko-Ippan-007 and Regulatory Science Research), JSPS KAKENHI (Grant Numbers 21406012 , 25305010 , 25893151 and 12J09845 ), and the Research Program on Emerging and Re-emerging Infectious Diseases from Japan Agency for Medical Research and development , AMED. The authors would like to thank Enago ( www.enago.jp ) for the English language review. Conflicts of interest: none.
Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2018/4/2
Y1 - 2018/4/2
N2 - The genus Thogotovirus, as represented by Thogoto virus and Dhori virus, comprises a group of arthropod-borne viruses, most members of which are transmitted by ticks. Here we report the genetic and biological characterization of a new thogotovirus, designated Oz virus (OZV), isolated from the hard tick Amblyomma testudinarium in Ehime, Japan. OZV efficiently replicated and induced a cytopathic effect in Vero cells, from which enveloped pleomorphic virus particles were formed by budding. OZV could also replicate in BHK-21 and DH82 cells and caused high mortality in suckling mice after intracerebral inoculation. Phylogenetic analyses of six viral proteins indicated that OZV is clustered with Dhori and related viruses, and is most closely related in glycoprotein (GP) and matrix protein (M) sequences to Bourbon virus, a human-pathogenic thogotovirus discovered recently in the United States. Our findings emphasize the need for understanding the geographic distribution and ecology of OZV and related viruses and for reevaluation of the medical and public health importance of thogotoviruses.
AB - The genus Thogotovirus, as represented by Thogoto virus and Dhori virus, comprises a group of arthropod-borne viruses, most members of which are transmitted by ticks. Here we report the genetic and biological characterization of a new thogotovirus, designated Oz virus (OZV), isolated from the hard tick Amblyomma testudinarium in Ehime, Japan. OZV efficiently replicated and induced a cytopathic effect in Vero cells, from which enveloped pleomorphic virus particles were formed by budding. OZV could also replicate in BHK-21 and DH82 cells and caused high mortality in suckling mice after intracerebral inoculation. Phylogenetic analyses of six viral proteins indicated that OZV is clustered with Dhori and related viruses, and is most closely related in glycoprotein (GP) and matrix protein (M) sequences to Bourbon virus, a human-pathogenic thogotovirus discovered recently in the United States. Our findings emphasize the need for understanding the geographic distribution and ecology of OZV and related viruses and for reevaluation of the medical and public health importance of thogotoviruses.
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U2 - 10.1016/j.virusres.2018.03.004
DO - 10.1016/j.virusres.2018.03.004
M3 - Article
C2 - 29548745
AN - SCOPUS:85044153609
SN - 0168-1702
VL - 249
SP - 57
EP - 65
JO - Virus Research
JF - Virus Research
ER -
