Characterization of a Caenorhabditis elegans recA-like gene Ce-rdh-1 involved in meiotic recombination

Takako Takanami; Shusei Sato; Takeshi Ishihara; Isao Katsura; Hideyuki Takahashi; Atsushi Higashitani

doi:10.1093/dnares/5.6.373

Characterization of a Caenorhabditis elegans recA-like gene Ce-rdh-1 involved in meiotic recombination

Takako Takanami, Shusei Sato, Takeshi Ishihara, Isao Katsura, Hideyuki Takahashi, Atsushi Higashitani

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

A recA-like gene was identified in the Caenorhabditis elegans genome project database. The putative product of the gene, termed Ce-rdh-1 (C. elegans RAD51 and DMC1/LIM15 homolog 1), consists of 357 amino acid residues. The predicted amino acid sequence of Ce-rdh-1 showed 46-60% identity to both RAD51 type and DMC1/LIM15 type genes in several eukaryote species. The results of RNAi (RNA-mediated interference) indicated that repression of Ce-rdh-1 blocked chromosome condensation of six bivalents and dissociation of chiasmata in oocytes of F₁ progeny. Oogenesis did not proceed to the diakinesis stage. Accordingly, all the eggs produced (F₂) died in early stages. These results suggest that Ce-rdh-1 participates in meiotic recombination.

Original language	English
Pages (from-to)	373-377
Number of pages	5
Journal	DNA Research
Volume	5
Issue number	6
DOIs	https://doi.org/10.1093/dnares/5.6.373
Publication status	Published - 1998
Externally published	Yes

All Science Journal Classification (ASJC) codes

Molecular Biology
Genetics

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10.1093/dnares/5.6.373

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title = "Characterization of a Caenorhabditis elegans recA-like gene Ce-rdh-1 involved in meiotic recombination",

abstract = "A recA-like gene was identified in the Caenorhabditis elegans genome project database. The putative product of the gene, termed Ce-rdh-1 (C. elegans RAD51 and DMC1/LIM15 homolog 1), consists of 357 amino acid residues. The predicted amino acid sequence of Ce-rdh-1 showed 46-60% identity to both RAD51 type and DMC1/LIM15 type genes in several eukaryote species. The results of RNAi (RNA-mediated interference) indicated that repression of Ce-rdh-1 blocked chromosome condensation of six bivalents and dissociation of chiasmata in oocytes of F1 progeny. Oogenesis did not proceed to the diakinesis stage. Accordingly, all the eggs produced (F2) died in early stages. These results suggest that Ce-rdh-1 participates in meiotic recombination.",

author = "Takako Takanami and Shusei Sato and Takeshi Ishihara and Isao Katsura and Hideyuki Takahashi and Atsushi Higashitani",

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T1 - Characterization of a Caenorhabditis elegans recA-like gene Ce-rdh-1 involved in meiotic recombination

AU - Takanami, Takako

AU - Sato, Shusei

AU - Ishihara, Takeshi

AU - Katsura, Isao

AU - Takahashi, Hideyuki

AU - Higashitani, Atsushi

PY - 1998

Y1 - 1998

N2 - A recA-like gene was identified in the Caenorhabditis elegans genome project database. The putative product of the gene, termed Ce-rdh-1 (C. elegans RAD51 and DMC1/LIM15 homolog 1), consists of 357 amino acid residues. The predicted amino acid sequence of Ce-rdh-1 showed 46-60% identity to both RAD51 type and DMC1/LIM15 type genes in several eukaryote species. The results of RNAi (RNA-mediated interference) indicated that repression of Ce-rdh-1 blocked chromosome condensation of six bivalents and dissociation of chiasmata in oocytes of F1 progeny. Oogenesis did not proceed to the diakinesis stage. Accordingly, all the eggs produced (F2) died in early stages. These results suggest that Ce-rdh-1 participates in meiotic recombination.

AB - A recA-like gene was identified in the Caenorhabditis elegans genome project database. The putative product of the gene, termed Ce-rdh-1 (C. elegans RAD51 and DMC1/LIM15 homolog 1), consists of 357 amino acid residues. The predicted amino acid sequence of Ce-rdh-1 showed 46-60% identity to both RAD51 type and DMC1/LIM15 type genes in several eukaryote species. The results of RNAi (RNA-mediated interference) indicated that repression of Ce-rdh-1 blocked chromosome condensation of six bivalents and dissociation of chiasmata in oocytes of F1 progeny. Oogenesis did not proceed to the diakinesis stage. Accordingly, all the eggs produced (F2) died in early stages. These results suggest that Ce-rdh-1 participates in meiotic recombination.

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Characterization of a Caenorhabditis elegans recA-like gene Ce-rdh-1 involved in meiotic recombination

Abstract

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