Characterization and treatment responsiveness of genetically engineered ornithine transcarbamylase-deficient pig

Shin Enosawa, Huai Che Hsu, Yusuke Yanagi, Hitomi Matsunari, Ayuko Uchikura, Hiroshi Nagashima

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

To develop novel medical technologies, pig disease models are invaluable especially in the final stages of translational research. Recently, we established a genetically engineered ornithine transcarbamylase-deficient (OTCD) pig strain. Here, we report its characterization and treatment responsiveness. OTCD pigs were obtained by mating an OTCD carrier female (OTC-Xc.186_190del XWT) with a wild-type male. Due to the X-linked recessive mode of inheritance, the disease phenotype emerged only in males. Medication with nitrogen-scavenging agents was based on a clinical protocol. OTCD pigs were born smaller than their wild-type and carrier littermates, showing anemia and faltering. Biochemically, high levels of urinary orotic acid and loss of OTC activity were observed. The natural life course of OTCD pigs was characterized by a decrease in arterial percentage saturation of oxygen and body temperature, as well as an increase in blood ammonia levels; the pigs died in 24.0 ± 5.0 h (mean ± SD, n = 6). The established standard medication composed with nitrogen-scavenging agents and transfusion nearly doubled the survival time to 42.4 ± 13.7 h (n = 6). Our OTCD pig model appropriately mimicked the human pathology. Along with established protocols in handling and medication, this is a first step in developing a large animal disease model that is useful for translational research into novel medical technologies, such as cell transplantation and gene therapy, as well as in relation to urea cycle disorder.

Original languageEnglish
Article number3226
JournalJournal of Clinical Medicine
Volume10
Issue number15
DOIs
Publication statusPublished - Aug 1 2021
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General Medicine

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