Changes in content of triterpenoids and polysaccharides in Ganoderma lingzhi at different growth stages

Toshinori Nakagawa, Qinchang Zhu, Sonam Tamrakar, Yhiya Amen, Yasuhiro Mori, Hiroto Suhara, Shuhei Kaneko, Hiroko Kawashima, Kotaro Okuzono, Yoshiyuki Inoue, Koichiro Ohnuki, Kuniyoshi Shimizu

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)


Ganoderma lingzhi is a traditional medicinal mushroom, and its extract contains many bioactive compounds. Triterpenoids and polysaccharides are the primary bioactive components that contribute to its medicinal properties. In this study, we quantified 18 triterpenoids, total triterpenoid content and total polysaccharide content in the ethanol and water extracts of G. lingzhi at different growth stages. Triterpenoids were quantified by liquid chromatograph–tandem mass spectrometry in the multiple-reaction-monitoring mode. Total triterpenoid and total polysaccharide content were determined by colorimetric analysis. The results indicated that the fruit bodies at an early growth stage had a higher content of ganoderic acid A, C2, I and LM2, as well as of ganoderenic acid C and D, than those at a later growth stage. In contrast, ganoderic acid K, TN and T–Q contents were higher in mature fruit bodies (maturation stage). The highest total triterpenoid and total polysaccharide contents were found in fruit bodies before maturity (stipe elongation stage or early stage of pileus formation). Our results provide information which will contribute to the establishment of an efficient cultivation system for G. lingzhi with a higher content of triterpenoids.

Original languageEnglish
Pages (from-to)734-744
Number of pages11
JournalJournal of Natural Medicines
Issue number3
Publication statusPublished - Jun 1 2018

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry


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