TY - JOUR
T1 - Cell-type specificity of human CYP11A1 TATA box
AU - Guo, Ing Cherng
AU - Chung, Bon Chu
N1 - Funding Information:
We thank Drs Feng-Fung Wang, Michael Uller, and Keith Parker for pCREtkCAT, pCMVc α , and the SF1-expression plasmid No. 289. This work was supported by Grant NSC85-2311-B001-034 from the National Science Council, and by Academia Sinica, Republic of China.
PY - 1999/4
Y1 - 1999/4
N2 - Expression of the CYP11A1 (SCC) genes, which encode the enzyme important for tile first step of steroid biosynthesis, occurs in the adrenal gland and gonads, and is stimulated by cAMP. Transfection of serial deletions of the SCC promoter, which drives reporter gene expression, showed that a minimal promoter containing only the TATA box could direct cAMP-dependent transcription. Transcription factor SF1, which binds to a site next to the TATA box, can stimulate basal transcription but not cAMP response, either in adrenal cell lines or in COS-1 co-transfected with the SF1 expression plasmid. These data lead to the conclusion that the minimal promoter containing only the TATA box can drive cell type-specific, cAMP-dependent transcription. Additional experiments replacing the TATA sequence of SCC with other TATA sequences suggested that the TATA sequence itself is important for this cAMP-dependent transcription.
AB - Expression of the CYP11A1 (SCC) genes, which encode the enzyme important for tile first step of steroid biosynthesis, occurs in the adrenal gland and gonads, and is stimulated by cAMP. Transfection of serial deletions of the SCC promoter, which drives reporter gene expression, showed that a minimal promoter containing only the TATA box could direct cAMP-dependent transcription. Transcription factor SF1, which binds to a site next to the TATA box, can stimulate basal transcription but not cAMP response, either in adrenal cell lines or in COS-1 co-transfected with the SF1 expression plasmid. These data lead to the conclusion that the minimal promoter containing only the TATA box can drive cell type-specific, cAMP-dependent transcription. Additional experiments replacing the TATA sequence of SCC with other TATA sequences suggested that the TATA sequence itself is important for this cAMP-dependent transcription.
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U2 - 10.1016/S0960-0760(99)00080-1
DO - 10.1016/S0960-0760(99)00080-1
M3 - Article
C2 - 10419010
AN - SCOPUS:0033022318
SN - 0960-0760
VL - 69
SP - 329
EP - 334
JO - Journal of Steroid Biochemistry and Molecular Biology
JF - Journal of Steroid Biochemistry and Molecular Biology
IS - 1-6
ER -
