TY - JOUR
T1 - Cell Surface-Anchored Fluorescent Probe Capable of Real-Time Imaging of Single Mast Cell Degranulation Based on Histamine-Induced Coordination Displacement
AU - Oshikawa, Yuji
AU - Furuta, Kazuyuki
AU - Tanaka, Satoshi
AU - Ojida, Akio
N1 - Publisher Copyright:
© 2016 American Chemical Society.
PY - 2016/2/2
Y1 - 2016/2/2
N2 - Mast cells secrete histamine upon degranulation triggered by various stimuli. Herein, we report the new detection method of mast cell degranulation using the fluorescent probe capable of detection of the released histamine. The probe was designed as the Co(II) complex of a cyanine dye, which shows a turn-on fluorescence signal based on a histamine-induced coordination displacement mechanism. Fluorescence imaging using the cell surface-anchored fluorescent probe enabled the real-time detection of mast cell degranulation induced by various secretagogues.
AB - Mast cells secrete histamine upon degranulation triggered by various stimuli. Herein, we report the new detection method of mast cell degranulation using the fluorescent probe capable of detection of the released histamine. The probe was designed as the Co(II) complex of a cyanine dye, which shows a turn-on fluorescence signal based on a histamine-induced coordination displacement mechanism. Fluorescence imaging using the cell surface-anchored fluorescent probe enabled the real-time detection of mast cell degranulation induced by various secretagogues.
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U2 - 10.1021/acs.analchem.5b04758
DO - 10.1021/acs.analchem.5b04758
M3 - Article
C2 - 26790581
AN - SCOPUS:84957583107
SN - 0003-2700
VL - 88
SP - 1526
EP - 1529
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 3
ER -