Cell-specific hypomethylation of the pepsinogen gene in pepsinogen-producing cells

Masao Ichinose; Kazumasa Miki; Masae Tatematsu; Takakazu Mizuno; Mamoru Mutai; Chie Furihata; Yoshikazu Ichihara; Takeshi Ishihara; Masao Tanji; Hiroshi Oka; Yoshikazu Hinohara; Takayuki Takahashi; Takashi Kageyama; Kenji Takahashi

doi:10.1016/S0006-291X(88)80547-3

Cell-specific hypomethylation of the pepsinogen gene in pepsinogen-producing cells

Masao Ichinose, Kazumasa Miki, Masae Tatematsu, Takakazu Mizuno, Mamoru Mutai, Chie Furihata, Yoshikazu Ichihara, Takeshi Ishihara, Masao Tanji, Hiroshi Oka, Yoshikazu Hinohara, Takayuki Takahashi, Takashi Kageyama, Kenji Takahashi

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The pepsinogen gene is hypomethylated in the stomach, in which it is expressed. For demonstration that this hypomethylation of the pepsinogen gene in the stomach reflects pepsinogen-producing cells, we analyzed fractions of dispersed mucosal cells with various contents of pepsinogen-producing cells prepared from guinea pig stomach by centrifugal elutriation. mRNA expression and the extent of hypomethylation of the pepsinogen gene in each fraction was closely correlated with the content of pepsinogen-producing cells. These results suggested hypomethylation of the pepsinogen gene in pepsinogen-producing cells and differential pepsinogen gene methylation in cell subpopulations in the stomach.

Original language	English
Pages (from-to)	670-677
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	155
Issue number	2
DOIs	https://doi.org/10.1016/S0006-291X(88)80547-3
Publication status	Published - Sept 15 1988
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/S0006-291X(88)80547-3

Cite this

Ichinose, M., Miki, K., Tatematsu, M., Mizuno, T., Mutai, M., Furihata, C., Ichihara, Y., Ishihara, T., Tanji, M., Oka, H., Hinohara, Y., Takahashi, T., Kageyama, T., & Takahashi, K. (1988). Cell-specific hypomethylation of the pepsinogen gene in pepsinogen-producing cells. Biochemical and Biophysical Research Communications, 155(2), 670-677. https://doi.org/10.1016/S0006-291X(88)80547-3

Ichinose, M, Miki, K, Tatematsu, M, Mizuno, T, Mutai, M, Furihata, C, Ichihara, Y, Ishihara, T, Tanji, M, Oka, H, Hinohara, Y, Takahashi, T, Kageyama, T & Takahashi, K 1988, 'Cell-specific hypomethylation of the pepsinogen gene in pepsinogen-producing cells', Biochemical and Biophysical Research Communications, vol. 155, no. 2, pp. 670-677. https://doi.org/10.1016/S0006-291X(88)80547-3

@article{353686c1eaad4c51a68d2b47dfb7eda7,

title = "Cell-specific hypomethylation of the pepsinogen gene in pepsinogen-producing cells",

abstract = "The pepsinogen gene is hypomethylated in the stomach, in which it is expressed. For demonstration that this hypomethylation of the pepsinogen gene in the stomach reflects pepsinogen-producing cells, we analyzed fractions of dispersed mucosal cells with various contents of pepsinogen-producing cells prepared from guinea pig stomach by centrifugal elutriation. mRNA expression and the extent of hypomethylation of the pepsinogen gene in each fraction was closely correlated with the content of pepsinogen-producing cells. These results suggested hypomethylation of the pepsinogen gene in pepsinogen-producing cells and differential pepsinogen gene methylation in cell subpopulations in the stomach.",

author = "Masao Ichinose and Kazumasa Miki and Masae Tatematsu and Takakazu Mizuno and Mamoru Mutai and Chie Furihata and Yoshikazu Ichihara and Takeshi Ishihara and Masao Tanji and Hiroshi Oka and Yoshikazu Hinohara and Takayuki Takahashi and Takashi Kageyama and Kenji Takahashi",

year = "1988",

month = sep,

day = "15",

doi = "10.1016/S0006-291X(88)80547-3",

language = "English",

volume = "155",

pages = "670--677",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Cell-specific hypomethylation of the pepsinogen gene in pepsinogen-producing cells

AU - Ichinose, Masao

AU - Miki, Kazumasa

AU - Tatematsu, Masae

AU - Mizuno, Takakazu

AU - Mutai, Mamoru

AU - Furihata, Chie

AU - Ichihara, Yoshikazu

AU - Ishihara, Takeshi

AU - Tanji, Masao

AU - Oka, Hiroshi

AU - Hinohara, Yoshikazu

AU - Takahashi, Takayuki

AU - Kageyama, Takashi

AU - Takahashi, Kenji

PY - 1988/9/15

Y1 - 1988/9/15

N2 - The pepsinogen gene is hypomethylated in the stomach, in which it is expressed. For demonstration that this hypomethylation of the pepsinogen gene in the stomach reflects pepsinogen-producing cells, we analyzed fractions of dispersed mucosal cells with various contents of pepsinogen-producing cells prepared from guinea pig stomach by centrifugal elutriation. mRNA expression and the extent of hypomethylation of the pepsinogen gene in each fraction was closely correlated with the content of pepsinogen-producing cells. These results suggested hypomethylation of the pepsinogen gene in pepsinogen-producing cells and differential pepsinogen gene methylation in cell subpopulations in the stomach.

AB - The pepsinogen gene is hypomethylated in the stomach, in which it is expressed. For demonstration that this hypomethylation of the pepsinogen gene in the stomach reflects pepsinogen-producing cells, we analyzed fractions of dispersed mucosal cells with various contents of pepsinogen-producing cells prepared from guinea pig stomach by centrifugal elutriation. mRNA expression and the extent of hypomethylation of the pepsinogen gene in each fraction was closely correlated with the content of pepsinogen-producing cells. These results suggested hypomethylation of the pepsinogen gene in pepsinogen-producing cells and differential pepsinogen gene methylation in cell subpopulations in the stomach.

UR - http://www.scopus.com/inward/record.url?scp=0023735968&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023735968&partnerID=8YFLogxK

U2 - 10.1016/S0006-291X(88)80547-3

DO - 10.1016/S0006-291X(88)80547-3

M3 - Article

C2 - 2458721

AN - SCOPUS:0023735968

SN - 0006-291X

VL - 155

SP - 670

EP - 677

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Cell-specific hypomethylation of the pepsinogen gene in pepsinogen-producing cells

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this