TY - JOUR
T1 - Cell adhesion on glassy scaffolds with a different mechanical response
AU - Shimomura, Shinichiro
AU - Matsuno, Hisao
AU - Sanada, Kazuaki
AU - Tanaka, Keiji
N1 - Funding Information:
This research was partly supported by JSPS KAKENHI, Grant-in-Aids for Scientific Research (A) (15H02183) to KT and Scientific Research (C) (15K05633) to HM. We also acknowledge the support from the JST SENTANKEISOKU (13A0004) to KT. We express our sincere thanks to Dr Tomoyasu Hirai of Kyushu University for his fruitful discussions, especially on the synthesis of PI using an anionic polymerization method.
Publisher Copyright:
© The Royal Society of Chemistry.
PY - 2017
Y1 - 2017
N2 - L929 mouse fibroblast cells were cultured on bilayer films composed of a glassy poly(methyl methacrylate) (PMMA) on a rubbery polyisoprene. When the thickness of the upper PMMA film fell short of a threshold value of 50 nm, the adhesion of fibroblasts on it was remarkably suppressed. A possible explanation is that the surface of a bilayer with an ultrathin PMMA layer apparently becomes softer due to the manifestation of a mechanical response from the rubbery layer underneath. Finite element analysis shows that the shear stress at the bilayer surface induced by traction force of the attached cells is dependent on the PMMA thickness, similar to the cell adhesion behavior. These results make it clear that fibroblasts can sense the surface stiffness of polymers with a modulus even on the order of MPa.
AB - L929 mouse fibroblast cells were cultured on bilayer films composed of a glassy poly(methyl methacrylate) (PMMA) on a rubbery polyisoprene. When the thickness of the upper PMMA film fell short of a threshold value of 50 nm, the adhesion of fibroblasts on it was remarkably suppressed. A possible explanation is that the surface of a bilayer with an ultrathin PMMA layer apparently becomes softer due to the manifestation of a mechanical response from the rubbery layer underneath. Finite element analysis shows that the shear stress at the bilayer surface induced by traction force of the attached cells is dependent on the PMMA thickness, similar to the cell adhesion behavior. These results make it clear that fibroblasts can sense the surface stiffness of polymers with a modulus even on the order of MPa.
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U2 - 10.1039/c6tb02617f
DO - 10.1039/c6tb02617f
M3 - Article
C2 - 32263839
AN - SCOPUS:85010736961
SN - 2050-7518
VL - 5
SP - 714
EP - 719
JO - Journal of Materials Chemistry B
JF - Journal of Materials Chemistry B
IS - 4
ER -