TY - JOUR
T1 - CDNA cloning of human calpastatin
T2 - Sequence homology among human, pig, and rabbit calpastatins
AU - Asada, Kiyozo
AU - Ishino, Yoshizumi
AU - Shimada, Masamitsu
AU - Shimojo, Tomoko
AU - Endo, Masahiro
AU - Kimizuka, Fusao
AU - Kato, Ikunoshin
AU - Maki, Masatoshi
AU - Hatanaka, Masakazu
AU - Murachi, Takashi
PY - 1989
Y1 - 1989
N2 - cDNA of human calpastatin, an inhibitor protein specific for calpain (EC3.4.22.17; Ca2+-dependent cysteine proteinase) was isolated by screening of a library prepared from human liver mRNA with pig calpastatin cDNA fragment as a probe. The primary stnkture of human calpastatin was deduced from the nucleotide sequence of the cDNA and compared with that of pig and rabbit calpastatins already reported. Human calpastatin consisted of 673 amino acid residues and had 78% and 77% indentity to pig or rabbit calpastatins. respectively. Human calpastatin had a domain structure with four internally repetitive sequences and one N-terminal non-homologous sequence like the other calpastatins. Human calpastatin had two deletions, 22 and 13 residues long in domain L and domain 1, respectively, compared to pig or rabbit calpastatins.
AB - cDNA of human calpastatin, an inhibitor protein specific for calpain (EC3.4.22.17; Ca2+-dependent cysteine proteinase) was isolated by screening of a library prepared from human liver mRNA with pig calpastatin cDNA fragment as a probe. The primary stnkture of human calpastatin was deduced from the nucleotide sequence of the cDNA and compared with that of pig and rabbit calpastatins already reported. Human calpastatin consisted of 673 amino acid residues and had 78% and 77% indentity to pig or rabbit calpastatins. respectively. Human calpastatin had a domain structure with four internally repetitive sequences and one N-terminal non-homologous sequence like the other calpastatins. Human calpastatin had two deletions, 22 and 13 residues long in domain L and domain 1, respectively, compared to pig or rabbit calpastatins.
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U2 - 10.3109/14756368909030363
DO - 10.3109/14756368909030363
M3 - Article
C2 - 2577276
AN - SCOPUS:0024383218
SN - 1475-6366
VL - 3
SP - 49
EP - 56
JO - Journal of Enzyme Inhibition and Medicinal Chemistry
JF - Journal of Enzyme Inhibition and Medicinal Chemistry
IS - 1
ER -