TY - JOUR
T1 - cDNA cloning of a wounding-inducible gene encoding a plastid ω-3 fatty acid desaturase from tobacco
AU - Hamada, Tatsurou
AU - Nishiuchi, Takumi
AU - Kodama, Hiroaki
AU - Nishimura, Mitsuo
AU - Iba, Koh
N1 - Funding Information:
The authors are grateful to Drs. Kaoru Suzuki and Hideaki Shinshi, National Institute of Bioscience and Human Technology, for providing us with a tobacco DAHP synthase cDNA. This research was supported in part by a Grant-in-Aid for Scientific Research on Priority Areas (no. 07251214 to K.I.) from the Ministry of Education, Science and Culture, Japan, and in part by the Cosmetology Research Foundation. T.H. and T.N. are the recipients of a scholarship from the Japan Society for the Promotion of Science.
PY - 1996/7
Y1 - 1996/7
N2 - A cDNA encoding the plastid ω-3 fatty acid desaturase was isolated from a tobacco (Nicotiana tabacum cv. SR1) leaf cDNA library. The amino terminal extension of the deduced amino acid sequence of this clone had a characteristic feature of the transit peptides of plastid-destined proteins. Northern analysis indicated that the mRNA corresponding to this cDNA was present in leaves, but was not detected in roots. Responses to wounding of the plastid and microsome ω-3 desaturase genes were investigated in tobacco leaves. The mRNA level of the plastid ω-3 desaturase gene increased to about 2-fold that of unwounded controls at 12 h after a wounding treatment. On the other hand, the mRNA level of the microsome ω-3 desaturase gene remained constant in the wounded leaves. Linolenic acid contents of major leaf polar lipids increased by wounding. These results indicate that wounding enhances the accumulation of the plastid ω-3 desaturase mRNA, and increases the conversion of linoleic acid to linolenic acid in leaf polar lipids.
AB - A cDNA encoding the plastid ω-3 fatty acid desaturase was isolated from a tobacco (Nicotiana tabacum cv. SR1) leaf cDNA library. The amino terminal extension of the deduced amino acid sequence of this clone had a characteristic feature of the transit peptides of plastid-destined proteins. Northern analysis indicated that the mRNA corresponding to this cDNA was present in leaves, but was not detected in roots. Responses to wounding of the plastid and microsome ω-3 desaturase genes were investigated in tobacco leaves. The mRNA level of the plastid ω-3 desaturase gene increased to about 2-fold that of unwounded controls at 12 h after a wounding treatment. On the other hand, the mRNA level of the microsome ω-3 desaturase gene remained constant in the wounded leaves. Linolenic acid contents of major leaf polar lipids increased by wounding. These results indicate that wounding enhances the accumulation of the plastid ω-3 desaturase mRNA, and increases the conversion of linoleic acid to linolenic acid in leaf polar lipids.
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U2 - 10.1093/oxfordjournals.pcp.a028988
DO - 10.1093/oxfordjournals.pcp.a028988
M3 - Article
C2 - 8819308
AN - SCOPUS:0030199033
SN - 0032-0781
VL - 37
SP - 606
EP - 611
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
IS - 5
ER -